Cell Adhesion-Mediated Piezoelectric Self-Stimulation on Polydopamine-Modified Poly (vinylidene fluoride) Membranes

被引：51
作者：
Xue, Guilan [1 ]
Zhang, Yimeng [1 ]
Xie, Tianpeng [1 ]
Zhang, Zhanlin [1 ]
Liu, Qingjie [1 ]
Li, Xiaohong [1 ]
Gou, Xue [1 ]
机构：
[1] Southwest Jiaotong Univ, Sch Mat Sci & Engn, Key Lab Adv Technol Mat, Minist Educ, Chengdu 610031, Sichuan, Peoples R China
来源：
ACS APPLIED MATERIALS & INTERFACES | 2021年 / 13卷 / 15期
基金：
中国国家自然科学基金;
关键词：
hydrophilicity; piezoelectricity; cell adhesion; self-stimulation; finite element simulation; POLY(VINYLIDENE FLUORIDE); ELECTRICAL-STIMULATION; PROLIFERATION; DIFFERENTIATION; BEHAVIOR; PHASES; FORCES;
D O I：
10.1021/acsami.1c02457
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Cell adhesion-mediated piezoelectric stimulation provides a noninvasive method for in situ electrical regulation of cell behavior, offering new opportunities for the design of smart materials for tissue engineering and bioelectronic medicines. In particular, the surface potential is mainly dominated by the inherent piezoelectricity of the biomaterial and the dynamic adhesion state of cells. The development of an efficient and optimized material interface would have important implications in cell regulation. Herein, we modified the surface of poled poly (vinylidene fluoride) (PVDF) membranes through polymerization of dopamine and investigated their influence on cell adhesion and electromechanical self-stimulation. Our results demonstrated that mesenchymal stem cells seeded on the poled PVDF membrane exhibited stronger cell spreading and adhesion. Meanwhile, the surface modification through polydopamine significantly improved the hydrophilicity of the samples and contributed to the formation of cell actin bundles and maturation of focal adhesions, which further positively modulated cell piezoelectric self-stimulation and induced intracellular calcium transients. Combining with theoretical simulations, we found that the self-stimulation was enhanced mainly due to the increase of the adhesion site and adhesion force magnitude. These findings provide new insights for probing the cell regulation mechanism on piezoelectric substrates, offering more opportunities for the rational design of piezoelectric biomaterial interfaces for biomedical engineering.
引用
收藏
页码：17361 / 17371
页数：11
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