Transformation of the entomopathogenic fungus, Metarhizium flavoviride strain CG423 to benomyl resistance

被引：13
|
作者：
Valadares-Inglis, MC [1 ]
Inglis, PW [1 ]
机构：
[1] CEN ARGEN, EMBRAPA, Biol Control Area ACB, BR-70849970 Brasilia, DF, Brazil
来源：
FEMS MICROBIOLOGY LETTERS | 1997年 / 155卷 / 02期
关键词：
biological control; grasshopper; transformation; benomyl; fungicide resistance; Metarhizium flavoviride;
D O I：
10.1111/j.1574-6968.1997.tb13878.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Metarhizium flavoviride strain CG423 is being developed as a mycoinsecticide against grasshoppers. This strain has been transformed to resistance to the fungicide benomyl by a polyethylene glycol (PEG)-mediated procedure using a mutant tubulin from Neurospora crassa. Transformation frequencies of up to 84 transformants per microgram of transforming DNA were achieved. Benomyl-resistant transformants were obtained that could tolerate greater than 30 mu g ml(-1) benomyl. Southern blot analysis of genomic DNA. reveals that the mechanism of genetic transformation of all transformants was by homologous gene replacement of the beta-tubulin allele.
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收藏
页码：199 / 202
页数：4
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