Time-resolved fluoroimmunoassay for ractopamine in swine tissue

被引：52
|
作者：
Shen, Jianzhong
Zhang, Zhen [1 ]
Yao, Yan
Shi, Weimin
Liu, Yibing
Zhang, Suxia
机构：
[1] China Agr Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Beijing 100094, Peoples R China
[2] China Inst Atom Energy, Dept Isotope, Beijing 102413, Peoples R China
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 387卷 / 04期
关键词：
time-resolved fluoroimmunoassay; ractopamine; swine tissue;
D O I：
10.1007/s00216-006-1063-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study describes the development and validation of a time-resolved fluoroimmunoassay (TR-FIA) for screening ractopamine (RAC) in swine tissue. The method is based on the direct competitive-type immunoassay using europium-labeled anti-RAC monoclonal antibody as a tracer and RAC-ovalbumin as a solid-phase antigen. When RAC was spiked at levels of 1-10 mu g kg(-1), recoveries ranged from 88.2 to 118.5% for swine liver and muscle with coefficients of variation from 7.1 to 20.5%. The detection limit was 0.1 mu g kg(-1). The proposed TR-FIA method was applied to the determination of RAC in an actual residue study and the applicability was confirmed by liquid chromatography-tandem mass spectrometry.
引用
收藏
页码：1561 / 1564
页数：4
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