Comparison of the measurement of lysosomal hydrolase activity in mycoplasma-contaminated and non-contaminated human fibroblast cultures treated with mycoplasma removal agent

被引：4
作者：
Souza, Fernanda Timm Seabra
Sostruznik, Luana Souza
Scolari, Roberta Casagrande
de Castro, Karen Joana Maciel
Glugliani, Roberto
Coelho, Janice Carneiro
机构：
[1] Hosp Clin Porto Alegre, Serv Genet Med, BR-90035903 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, ICBS, Dept Bioquim, Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, IB, Dept Genet, Porto Alegre, RS, Brazil
来源：
CLINICAL BIOCHEMISTRY | 2007年 / 40卷 / 08期
关键词：
mycoplasma; lysosomal hydrolase; lysosomal storage diseases; fibroblast cultures;
D O I：
10.1016/j.clinbiochem.2007.01.016
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: To investigate the effect of both mycoplasma contamination and of its remover (MRA), through human fibroblasts Culture over the activity of some lysosomal hydrolases. Design and methods: Activity was measured in contaminated fibroblasts before and after the addition of MRA. Results were compared with the enzymatic activity in control fibroblasts with and without MRA. Results: Only beta-glucosidase showed no significant alteration in the presence of either mycoplasma or MRA. Total hexosaminidase and galactosidase underwent significant interference in the presence of the mycoplasma and the MRA. The % of hexosaminidase A and arylsulphatase A altered their activity only in the presence of MRA. beta-Glucuronidase changed its activity only in the presence of mycoplasma. Conclusions: The fibroblast enzymes behaved differently in the presence of MRA and/or mycoplasma, demonstrating the sensitivity of these hydrolases. Our work suggests that mycoplasma and MRA after the activity of some lysosonial hydrolases. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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页码：521 / 525
页数：5
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