AChE biosensor based on zinc oxide sol-gel for the detection of pesticides

被引：61

作者：

Sinha, Ravi ^{[2
]}

Ganesana, Mallikarjunarao ^{[1
]}

Andreescu, Silvana ^{[1
]}

Stanciu, Lia ^{[2
]}

机构：

[1] Clarkson Univ, Dept Chem, Potsdam, NY 13699 USA

[2] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA

来源：

ANALYTICA CHIMICA ACTA | 2010年 / 661卷 / 02期

基金：

美国国家科学基金会;

关键词：

Zinc oxide; Sol-gel; Pesticide biosensor; Paraoxon; Acetylcholinesterase; SCREEN-PRINTED ELECTRODES; ELECTROCHEMICAL BIOSENSORS; ACETYLCHOLINESTERASE; IMMOBILIZATION; MATRIX; ENCAPSULATION; COMPOSITE; CONSTRUCTION; MEMBRANE; SYSTEM;

D O I：

10.1016/j.aca.2009.12.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Zinc oxide has been used as a matrix for immobilization of acetylcholinesterase (AChE) and detection of the pesticide paraoxon. The immobilized enzyme retained its enzymatic activity up to three months when stored in phosphate buffered saline (pH 7.4) at 4 degrees C. An amperometric biosensor for the detection of paraoxon was designed. The biosensor detected paraoxon in the range 0.035-1.38 ppm and can be used to detect other AChE inhibiting organophosphate pesticides. (C) 2009 Elsevier B.V. All rights reserved.

引用

收藏

页码：195 / 199

页数：5

相关论文

共 32 条

[1] Ultrasensitive detection of cytokines enabled by nanoscale ZnO arrays [J].

Adalsteinsson, Viktor ;

Parajuli, Omkar ;

Kepics, Stephen ;

Gupta, Abhishek ;

Reeves, W. Brian ;

Hahm, Jong-in .

ANALYTICAL CHEMISTRY, 2008, 80 (17) :6594-6601

[2] Investigation of organic phase biosensor for measuring glucose in flow injection analysis system [J].

Adányi, N ;

Tóth-Markus, M ;

Szabó, EE ;

Váradi, M ;

Sammartino, MP ;

Tomassetti, M ;

Campanella, L .

ANALYTICA CHIMICA ACTA, 2004, 501 (02) :219-225

[3] Preliminary estimates of lactic and malic acid in wine using electrodes printed from inks containing sol-gel precursors [J].

Albareda-Sirvent, M ;

Hart, AL .

SENSORS AND ACTUATORS B-CHEMICAL, 2002, 87 (01) :73-81

[4] Twenty years research in cholinesterase biosensors: From basic research to practical applications [J].

Andreescu, S ;

Marty, JL .

BIOMOLECULAR ENGINEERING, 2006, 23 (01) :1-15

[5] Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides [J].

Andreescu, S ;

Barthelmebs, L ;

Marty, JL .

ANALYTICA CHIMICA ACTA, 2002, 464 (02) :171-180

[6] Development of acetylcholinesterase silica sol-gel immobilized biosensor - an application towards oxydemeton methyl detection [J].

Anitha, K ;

Mohan, SV ;

Reddy, SJ .

BIOSENSORS & BIOELECTRONICS, 2004, 20 (04) :848-856

[7] Electrocatalytic oxidation of thiocholine at chemically modified cobalt hexacyanoferrate screen-printed electrodes [J].

Arduini, Fabiana ;

Cassisi, Alessio ;

Amine, Aziz ;

Ricci, Francesco ;

Moscone, Danila ;

Palleschi, Giuseppe .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2009, 626 (1-2) :66-74

[8] BIOCHEMICALLY ACTIVE SOL-GEL GLASSES - THE TRAPPING OF ENZYMES [J].

BRAUN, S ;

RAPPOPORT, S ;

ZUSMAN, R ;

AVNIR, D ;

OTTOLENGHI, M .

MATERIALS LETTERS, 1990, 10 (1-2) :1-5

[9] Spectrophotometric determination of enzymatically generated hydrogen peroxide using Sol-Gel immobilized horseradish peroxidase [J].

ElEssi, FA ;

AbuZuhri, AZ ;

AlKhalil, SI ;

AbdelLatif, MS .

TALANTA, 1997, 44 (11) :2051-2058

[10] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].

ELLMAN, GL ;

COURTNEY, KD ;

ANDRES, V ;

FEATHERSTONE, RM .

BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&

← 1 2 3 4 →