Multistep involvement of glutathione with salicylic acid and ethylene to combat environmental stress

被引:51
作者
Ghanta, Srijani [1 ]
Datta, Riddhi [1 ]
Bhattacharyya, Dipto [1 ]
Sinha, Ragini [1 ]
Kumar, Deepak [1 ]
Hazra, Saptarshi [1 ]
Mazumdar, Aparupa Bose [1 ]
Chattopadhyay, Sharmila [1 ]
机构
[1] CSIR, Indian Inst Chem Biol, Plant Biol Lab, Drug Dev Diagnost & Biotechnol Div, Kolkata 700032, India
关键词
Defense signaling network; Ethylene; Glutathione; Salicylic acid; Stress; DEPENDENT DEFENSE-RESPONSE; GAMMA-GLUTAMYLCYSTEINE; TRANSCRIPTION FACTORS; ACQUIRED-RESISTANCE; ARABIDOPSIS; TOBACCO; GENE; BIOSYNTHESIS; METABOLISM; EXPRESSION;
D O I
10.1016/j.jplph.2014.03.002
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The role of glutathione (GSH) in plant defense is an established fact. However, the association of GSH with other established signaling molecules within the defense signaling network remains to be evaluated. Previously we have shown that GSH is involved in defense signaling network likely through NPR1-dependent salicylic acid (SA)-mediated pathway. In this study, to gain further insight, we developed chloroplast-targeted gamma-glutamylcysteine synthetase (gamma-ECS) overexpressed transgenic Nicotiana tabacum (NtGp line) and constructed a forward subtracted cDNA (suppression subtractive hybridization (SSH)) library using NtGp line as a tester. Interestingly, in addition to SA-related transcripts like pathogenesis-related protein 1a (PR1a) and SAR8.2 m/2l, 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase), a key enzyme of ethylene (ET) biosynthesis, was identified in the SSH library. Besides, transcription factors like WRKY transcription factor 3 (WRKY3), WRKY1 and ethylene responsive factor 4 (ERF4), associated with SA and ET respectively, were also identified thus suggesting an interplay of GSH with ET and SA. Furthermore, proteomic profiling of NtGp line, performed by employing two-dimensional gel electrophoresis (2-DE), corroborated with the transcriptomic profile and several defense-related proteins like serine/threonine protein kinase, and heat shock 70 protein (HSP70) were identified with increased accumulation. Fascinatingly, induction of 1-aminocyclopropane-1-carboxylate synthase (ACC synthase) was also noted thus demonstrating the active involvement of GSH with ET. Protein gel blot analysis confirmed the enhanced accumulation of ACC oxidase in NtGp line. Together, our data revealed that GSH is involved in the synergistic multiple steps crosstalk through ET as well as SA to combat environmental stress. (C) 2014 Elsevier GmbH. All rights reserved.
引用
收藏
页码:940 / 950
页数:11
相关论文
共 60 条
[1]   A NEW MULTIGENE FAMILY INDUCIBLE BY TOBACCO MOSAIC-VIRUS OR SALICYLIC-ACID IN TOBACCO [J].
ALEXANDER, D ;
STINSON, J ;
PEAR, J ;
GLASCOCK, C ;
WARD, E ;
GOODMAN, RM ;
RYALS, J .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1992, 5 (06) :513-515
[2]   THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES [J].
BJELLQVIST, B ;
HUGHES, GJ ;
PASQUALI, C ;
PAQUET, N ;
RAVIER, F ;
SANCHEZ, JC ;
FRUTIGER, S ;
HOCHSTRASSER, D .
ELECTROPHORESIS, 1993, 14 (10) :1023-1031
[3]   Molecular chaperones and protein folding in plants [J].
Boston, RS ;
Viitanen, PV ;
Vierling, E .
PLANT MOLECULAR BIOLOGY, 1996, 32 (1-2) :191-222
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID USING ORTHO-PHTHALDIALDEHYDE PRECOLUMN DERIVATIZATION [J].
BUSHEY, DF ;
LAW, DM ;
DAVIES, PJ .
ANALYTICAL BIOCHEMISTRY, 1987, 167 (01) :31-36
[6]   Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress [J].
Creissen, G ;
Firmin, J ;
Fryer, M ;
Kular, B ;
Leyland, N ;
Reynolds, H ;
Pastori, G ;
Wellburn, F ;
Baker, N ;
Wellburn, A ;
Mullineaux, P .
PLANT CELL, 1999, 11 (07) :1277-1291
[7]  
Datta R, 2013, J BIOSCIENCE, V38, P1
[8]   Herbivore-induced resistance against microbial pathogens in Arabidopsis [J].
De Vos, Martin ;
Van Zaanen, Wendy ;
Koornneef, Annemart ;
Korzelius, Jerome P. ;
Dicke, Marcel ;
Van Loon, L. C. ;
Pieterse, Corne M. J. .
PLANT PHYSIOLOGY, 2006, 142 (01) :352-363
[9]   GLUTATHIONE AND FUNGAL ELICITOR REGULATION OF A PLANT DEFENSE GENE PROMOTER IN ELECTROPORATED PROTOPLASTS [J].
DRON, M ;
CLOUSE, SD ;
DIXON, RA ;
LAWTON, MA ;
LAMB, CJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6738-6742
[10]   MYB transcription factors in Arabidopsis [J].
Dubos, Christian ;
Stracke, Ralf ;
Grotewold, Erich ;
Weisshaar, Bernd ;
Martin, Cathie ;
Lepiniec, Loic .
TRENDS IN PLANT SCIENCE, 2010, 15 (10) :573-581