What is the best ethanol-water ratio for the extraction of antioxidants from rosemary? Impact of the solvent on yield, composition, and activity of the extracts

被引：51
作者：
Jacotet-Navarro, Magali [1 ,2 ,3 ]
Laguerre, Mickael [3 ]
Fabiano-Tixier, Anne-Sylvie [1 ,2 ]
Tenon, Mathieu [3 ]
Feuillere, Nicolas [3 ]
Bily, Antoine [2 ,3 ]
Chemat, Farid [1 ,2 ]
机构：
[1] Univ Avignon, INRA, UMR408, GREEN Extract Team, Avignon, France
[2] Univ Avignon, ORTESA, LabCom Naturex, Avignon, France
[3] Naturex, Avignon, France
来源：
ELECTROPHORESIS | 2018年 / 39卷 / 15期
关键词：
Antioxidants; Carnosic acid; Extraction; Rosmarinic acid; Rosemary; Solvent selection; ROSMARINUS-OFFICINALIS L; CARNOSIC ACID; ESSENTIAL OIL; COSMO-RS; ASSISTED EXTRACTION; GREEN SOLVENTS; MICROWAVE; ULTRASOUND; PREDICTION; LEAVES;
D O I：
10.1002/elps.201700397
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Extracts rich in antioxidants, such as rosemary extracts, are currently obtained by extraction of the plant material using hydro-alcoholic mixtures with high ethanol content. As this ratio is generally chosen by default and scarcely optimized, we intended to investigate the impact of the hydro-alcoholic composition on extract characteristics such as extraction yield, composition profile in selected compounds, and antioxidant/reducing activity such as Folin-Ciocalteu, DPPH, and ORAC. A theoretical determination of rosmarinic (RA) and carnosic (CA) acid solubilities in ethanol:water mixtures was also performed using COSMO-RS and was confronted to experiments. While the best solubilizing solvent (100% ethanol) was also the best extracting solvent for CA, it was not the case with RA since pure ethanol appeared as a poor solvent compared to 30% ethanol which was optimal. Finally, the best antioxidant activities were obtained with 30% ethanol.
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页码：1946 / 1956
页数：11
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