Membrane protein megahertz crystallography at the European XFEL

被引：51

作者：

Gisriel, Chris ^{[1
,2
,23
]}

Coe, Jesse ^{[1
,2
]}

Letrun, Romain ^{[3
]}

Yefanov, Oleksandr M. ^{[4
]}

Luna-Chavez, Cesar ^{[1
,2
]}

Stander, Natasha E. ^{[1
,2
]}

Lisova, Stella ^{[1
,5
]}

Mariani, Valerio ^{[4
]}

Kuhn, Manuela ^{[4
]}

Aplin, Steve ^{[4
]}

Grant, Thomas D. ^{[6
,7
]}

Doerner, Katerina ^{[3
]}

Sato, Tokushi ^{[3
,4
]}

Echelmeier, Austin ^{[1
,2
]}

Villarreal, Jorvani Cruz ^{[1
,2
]}

Hunter, Mark S. ^{[8
]}

Wiedorn, Max O. ^{[4
,9
,10
]}

Knoska, Juraj ^{[4
]}

Mazalova, Victoria ^{[4
]}

Roy-Chowdhury, Shatabdi ^{[1
,2
]}

Yang, Jay-How ^{[1
,2
]}

Jones, Alex ^{[1
,2
]}

Bean, Richard ^{[3
]}

Bielecki, Johan ^{[3
]}

Kim, Yoonhee ^{[3
]}

Mills, Grant ^{[3
]}

Weinhausen, Britta ^{[3
]}

Meza, Jose D. ^{[3
]}

Al-Qudami, Nasser ^{[3
]}

Bajt, Sasa ^{[11
]}

Brehm, Gerrit ^{[1
,2
,12
,13
]}

Botha, Sabine ^{[5
]}

Boukhelef, Djelloul ^{[3
]}

Brockhauser, Sandor ^{[3
,14
]}

Bruce, Barry D. ^{[15
,16
,17
]}

Coleman, Matthew A. ^{[18
]}

Danilevski, Cyril ^{[3
]}

Discianno, Erin ^{[1
]}

Dobson, Zachary ^{[1
,2
]}

Fangohr, Hans ^{[3
,19
]}

Martin-Garcia, Jose M. ^{[1
]}

Gevorkov, Yaroslav ^{[4
,20
]}

Hauf, Steffen ^{[3
]}

Hosseinizadeh, Ahmad ^{[21
]}

Januschek, Friederike ^{[3
,24
]}

Ketawala, Gihan K. ^{[1
,2
]}

Kupitz, Christopher ^{[8
,21
]}

Maia, Luis ^{[3
]}

Manetti, Maurizio ^{[3
]}

Messerschmidt, Marc ^{[1
,2
,3
]}

机构：

[1] Arizona State Univ, Biodesign Ctr Appl Struct Discovery, Tempe, AZ 85287 USA

[2] Arizona State Univ, Sch Mol Sci, Tempe, AZ 85287 USA

[3] European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany

[4] DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany

[5] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[6] Hauptman Woodward Inst, 700 Ellicott St, Buffalo, NY 14203 USA

[7] SUNY Univ Buffalo, Jacobs Sch Med & Biomed Sci, Dept Biol Struct, 700 Ellicott St, Buffalo, NY 14203 USA

[8] SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA

[9] Univ Hamburg, Dept Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany

[10] Univ Hamburg, Hamburg Ctr Ultrafast Imaging, Luruper Chaussee 149, D-22761 Hamburg, Germany

[11] DESY, Notkestr 85, D-22607 Hamburg, Germany

[12] Univ Gottingen, Inst X Ray Phys, D-37077 Gottingen, Germany

[13] Ctr Nanoscale Microscopy & Mol Physiol Brain, Gottingen, Germany

[14] Hungarian Acad Sci, Biol Res Ctr, Temesvari Krt 62, H-6726 Szeged, Hungary

[15] Univ Tennessee, Dept Biochem & Cellular & Mol Biol, Knoxville, TN 37996 USA

[16] Univ Tennessee, Program Energy Sci & Engn, Knoxville, TN 37996 USA

[17] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA

[18] Lawrence Livermore Natl Lab, 7000 East Ave, Livermore, CA 94550 USA

[19] Univ Southampton, Univ Rd, Southampton SO17 1BJ, Hants, England

[20] Hamburg Univ Technol, Vis Syst E-2,Harburger Schlossstr 20, D-21079 Hamburg, Germany

[21] Univ Wisconsin, Dept Phys, 3135 N Maryland Ave, Milwaukee, WI 53211 USA

[22] La Trobe Univ, La Trobe Inst Mol Sci, Dept Chem & Phys, Melbourne, Vic 3086, Australia

[23] Yale Univ, Dept Chem, 225 Prospect St, New Haven, CT 06520 USA

[24] DESY, Notkestr 85, D-22607 Hamburg, Germany

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

澳大利亚研究理事会; 美国国家卫生研究院; 美国国家科学基金会; 欧洲研究理事会;

关键词：

PHOTOSYSTEM-I; SERIAL CRYSTALLOGRAPHY; SAMPLE DELIVERY; 3-DIMENSIONAL STRUCTURE; REFINEMENT; DETECTOR; MODEL;

D O I：

10.1038/s41467-019-12955-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The world's first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 angstrom-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.

引用

页数：11

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