A study of the interaction of rhodamine 6G with DNA by spectrophotometry and probe microscopy

被引:7
作者
Letuta, SN [1 ]
Ketsle, GA
Levshin, LV
Nikiyan, AN
Davydova, OK
机构
[1] Orenburg State Univ, Orenburg 460352, Russia
[2] Moscow MV Lomonosov State Univ, Fac Phys, Moscow 117234, Russia
关键词
Double Helix; Scanning Probe Microscopy; Exterior Surface; Cooperative Binding; Atomic Force Micro;
D O I
10.1134/1.1531706
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
The interaction of rhodamine 6G with DNA was studied by the methods of spectrophotometry and scanning probe microscopy. It was ascertained that rhodamine 6G interacts with the DNA macromolecules by cooperative binding to the exterior surface of the polymeric chain. As a result, organic nanostructures form on the surface of the polymers. The number of dye molecules in the nanostructures is estimated. The distribution of these structures along the double helix of DNA and their merging into larger conglomerates is studied. (C) 2002 MAIK "Nauka/Interperiodica".
引用
收藏
页码:844 / 847
页数:4
相关论文
共 19 条
[1]   Vladimir Vasil'evich Zheleznyakov (on his seventieth birthday) [J].
Alfimov, MV ;
Andreev, AF ;
Boyarchuk, AA ;
Gaponov-Grekhov, AV ;
Ginzburg, VL ;
Zaitsev, VV ;
Litvak, AG ;
Syunyaev, RA ;
Talanov, VI ;
Trakhtengerts, VY ;
Khanin, YI ;
Khokhlov, AF .
USPEKHI FIZICHESKIKH NAUK, 2001, 171 (02) :225-226
[2]  
BLAGOI YP, 1998, SOROS OBRAZ ZH, P24
[3]   CIRCULAR DNA-MOLECULES IMAGED IN AIR BY SCANNING FORCE MICROSCOPY [J].
BUSTAMANTE, C ;
VESENKA, J ;
TANG, CL ;
REES, W ;
GUTHOLD, M ;
KELLER, R .
BIOCHEMISTRY, 1992, 31 (01) :22-26
[4]  
CHAN EW, 1971, BIOCHIM BIOPHYS ACTA, V238, P663
[5]  
Chibisov A. K., 1975, Optics and Spectroscopy, V38, P45
[6]  
DUCHESNE J, 1973, PHYSICOCHEMICAL PROP
[7]  
GALLYAMOV MO, 1997, SCANNING SOUND MICRO
[8]  
IVANOV AV, 2000, LAZER INFORM, P206
[9]  
Krasnovskii A. A., 1990, ITOGI NAUKI TEK SPLF, V3, P63
[10]  
Levshin L. V., 1989, Luminescence and Its Measurements: Molecular Luminescence