Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients

被引：27

作者：

Yokota, Isao ^{[1
]}

Hattori, Takeshi ^{[2
]}

Shane, Peter Y. ^{[3
]}

Konno, Satoshi ^{[4
]}

Nagasaka, Atsushi ^{[5
]}

Takeyabu, Kimihiro ^{[6
]}

Fujisawa, Shinichi ^{[7
]}

Nishida, Mutsumi ^{[7
]}

Teshima, Takanori ^{[3
,7
,8
]}

机构：

[1] Hokkaido Univ, Dept Biostat, Fac Med, Sapporo, Hokkaido, Japan

[2] Natl Hosp Org, Dept Resp Med, Hokkaido Med Ctr, Sapporo, Hokkaido, Japan

[3] Hokkaido Univ Hosp, Int Med Dept, Sapporo, Hokkaido, Japan

[4] Hokkaido Univ, Dept Resp Med, Fac Med, Sapporo, Hokkaido, Japan

[5] Sapporo City Gen Hosp, Dept Infect Dis, Sapporo, Hokkaido, Japan

[6] Otaru Kyokai Hosp, Dept Resp Med, Otaru, Hokkaido, Japan

[7] Hokkaido Univ Hosp, Div Lab & Transfus Med, Sapporo, Hokkaido, Japan

[8] Hokkaido Univ, Dept Hematol, Fac Med, Kita Ku, N15 W7, Sapporo, Hokkaido 0608638, Japan

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

D O I：

10.1038/s41598-021-84059-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Emerging evidences have shown the utility of saliva for the detection of SARS-CoV-2 by PCR as alternative to nasopharyngeal swab (NPS). However, conflicting results have been reported regarding viral loads between NPS and saliva. We conducted a study to compare the viral loads between NPS and saliva in 42 COVID-19 patients. Viral loads were estimated by the cycle threshold (Ct) values. SARS-CoV-2 was detected in 34 (81%) using NPS with median Ct value of 27.4, and 38 (90%) using saliva with median Ct value of 28.9 (P=0.79). Kendall's W was 0.82, showing a high degree of agreement, indicating equivalent viral loads in NPS and saliva. After symptom onset, the Ct values of both NPS and saliva continued to increase over time, with no substantial difference. Self-collected saliva has a detection sensitivity comparable to that of NPS and is a useful diagnostic tool with mitigating uncomfortable process and the risk of aerosol transmission to healthcare workers.

引用

页数：4

共 9 条

[1] Saliva is a reliable tool to detect SARS-CoV-2 [J].

Azzi, Lorenzo ;

Carcano, Giulio ;

Gianfagna, Francesco ;

Grossi, Paolo ;

Dalla Gasperina, Daniela ;

Genoni, Angelo ;

Fasano, Mauro ;

Sessa, Fausto ;

Tettamanti, Lucia ;

Carinci, Francesco ;

Maurino, Vittorio ;

Rossi, Agostino ;

Tagliabue, Angelo ;

Baj, Andreina .

JOURNAL OF INFECTION, 2020, 81 (01) :E45-E50

[2] Comparison of SARS-CoV-2 detection in nasopharyngeal swab and saliva [J].

Iwasaki, Sumio ;

Fujisawa, Shinichi ;

Nakakubo, Sho ;

Kamada, Keisuke ;

Yamashita, Yu ;

Fukumoto, Tatsuya ;

Sato, Kaori ;

Oguri, Satoshi ;

Taki, Keisuke ;

Senjo, Hajime ;

Sugita, Junichi ;

Hayasaka, Kasumi ;

Konno, Satoshi ;

Nishida, Mutsumi ;

Teshima, Takanori .

JOURNAL OF INFECTION, 2020, 81 (02) :E145-E147

[3] Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription-Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19 [J].

Nagura-Ikeda, Mayu ;

Imai, Kazuo ;

Tabata, Sakiko ;

Miyoshi, Kazuyasu ;

Murahara, Nami ;

Mizuno, Tsukasa ;

Horiuchi, Midori ;

Kato, Kento ;

Imoto, Yoshitaka ;

Mimura, Satoshi ;

Ito, Toshimitsu ;

Tamura, Kaku ;

Kato, Yasuyuki .

JOURNAL OF CLINICAL MICROBIOLOGY, 2020, 58 (09)

[4] Consistent Detection of 2019 Novel Coronavirus in Saliva [J].

To, Kelvin Kai-Wang ;

Tsang, Owen Tak-Yin ;

Yip, Cyril Chik-Yan ;

Chan, Kwok-Hung ;

Wu, Tak-Chiu ;

Chan, Jacky Man-Chun ;

Leung, Wai-Shing ;

Chik, Thomas Shiu-Hong ;

Choi, Chris Yau-Chung ;

Kandamby, Darshana H. ;

Lung, David Christopher ;

Tam, Anthony Raymond ;

Poon, Rosana Wing-Shan ;

Fung, Agnes Yim-Fong ;

Hung, Ivan Fan-Ngai ;

Cheng, Vincent Chi-Chung ;

Chan, Jasper Fuk-Woo ;

Yuen, Kwok-Yung .

CLINICAL INFECTIOUS DISEASES, 2020, 71 (15) :841-843

[5] Swabs Collected by Patients or Health Care Workers for SARS-CoV-2 Testing [J].

Tu, Yuan-Po ;

Jennings, Rachel ;

Berke, Ethan M. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 383 (05) :494-496

[6] Detection of SARS-CoV-2 in Different Types of Clinical Specimens [J].

Wang, Wenling ;

Xu, Yanli ;

Gao, Ruqin ;

Lu, Roujian ;

Han, Kai ;

Wu, Guizhen ;

Tan, Wenjie .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2020, 323 (18) :1843-1844

[7] Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2 [J].

Williams, Eloise ;

Bond, Katherine ;

Zhang, Bowen ;

Putland, Mark ;

Williamson, Deborah A. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2020, 58 (08)

[8] Saliva or Nasopharyngeal Swab Specimens for Detection of SARS-CoV-2 [J].

Wyllie, Anne L. ;

Fournier, John ;

Casanovas-Massana, Arnau ;

Campbell, Melissa ;

Tokuyama, Maria ;

Vijayakumar, Pavithra ;

Warren, Joshua L. ;

Geng, Bertie ;

Muenker, M. Catherine ;

Moore, Adam J. ;

Vogels, Chantal B. F. ;

Petrone, Mary E. ;

Ott, Isabel M. ;

Lu, Peiwen ;

Venkataraman, Arvind ;

Lu-Culligan, Alice ;

Klein, Jonathan ;

Earnest, Rebecca ;

Simonov, Michael ;

Datta, Rupak ;

Handoko, Ryan ;

Naushad, Nida ;

Sewanan, Lorenzo R. ;

Valdez, Jordan ;

White, Elizabeth B. ;

Lapidus, Sarah ;

Kalinich, Chaney C. ;

Jiang, Xiaodong ;

Kim, Daniel J. ;

Kudo, Eriko ;

Linehan, Melissa ;

Mao, Tianyang ;

Moriyama, Miyu ;

Oh, Ji E. ;

Park, Annsea ;

Silva, Julio ;

Song, Eric ;

Takahashi, Takehiro ;

Taura, Manabu ;

Weizman, Orr-El ;

Wong, Patrick ;

Yang, Yexin ;

Bermejo, Santos ;

Odio, Camila D. ;

Omer, Saad B. ;

Dela Cruz, Charles S. ;

Farhadian, Shelli ;

Martinello, Richard A. ;

Iwasaki, Akiko ;

Grubaugh, Nathan D. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 383 (13) :1283-1286

[9] Mass Screening of Asymptomatic Persons for Severe Acute Respiratory Syndrome Coronavirus 2 Using Saliva [J].

Yokota, Isao ;

Shane, Peter Y. ;

Okada, Kazufumi ;

Unoki, Yoko ;

Yang, Yichi ;

Inao, Tasuku ;

Sakamaki, Kentaro ;

Iwasaki, Sumio ;

Hayasaka, Kasumi ;

Sugita, Junichi ;

Nishida, Mutsumi ;

Fujisawa, Shinichi ;

Teshima, Takanori .

CLINICAL INFECTIOUS DISEASES, 2021, 73 (03) :E559-E565

← 1 →