Development of a rapid bacterial counting method based on photothermal assembling

被引:31
作者
Yamamoto, Yasuyuki [1 ,2 ]
Shimizu, Emi [3 ]
Nishimura, Yushi [1 ]
Iida, Takuya [1 ]
Tokonami, Shiho [3 ]
机构
[1] Osaka Prefecture Univ, Grad Sch Sci, Dept Phys Sci, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan
[2] Osaka Prefecture Univ, Grad Sch Engn, Dept Phys & Elect, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan
[3] Osaka Prefecture Univ, Grad Sch Engn, Dept Appl Chem, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan
关键词
OPTICAL-FIBER; PARTICLES; RING; SOIL;
D O I
10.1364/OME.6.001280
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
We developed a rapid bacterial counting method based on the photothermal assembling (PTA). Based on the laser-induced PTA in fluid medium, an initial bacterial concentration was estimated from the number of assembled bacteria. The measuring time of our method is 90 s, which is more rapid than the conventional cultivation method requiring several days at longest. Furthermore, the difference between the estimated concentrations by our method and by the cultivation method is less than 10%, which sufficiently guarantees the availability. The clarified principle will pave the way to a rapid and high throughput bacterial assay useful for medical care and food safety. (C) 2016 Optical Society of America
引用
收藏
页码:1280 / 1285
页数:6
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