Sodium-Calcium Exchangers in Rat Ameloblasts

被引：41
作者：
Okumura, Reijiro [1 ,2 ,3 ]
Shibukawa, Yoshiyuki [1 ,4 ]
Muramatsu, Takashi [1 ,3 ]
Hashimoto, Sadamitsu [1 ,3 ]
Nakagawa, Kan-Ichi [2 ]
Tazaki, Masakazu [1 ,4 ]
Shimono, Masaki [3 ]
机构：
[1] Tokyo Dent Coll, Oral Hlth Sci Ctr, Hrc7, Mihama Ku, Chiba 2618502, Japan
[2] Tokyo Dent Coll, Dept Endodont Pulp & Periap Biol, Mihama Ku, Chiba 2618502, Japan
[3] Tokyo Dent Coll, Dept Pathol, Mihama Ku, Chiba 2618502, Japan
[4] Tokyo Dent Coll, Dept Physiol, Mihama Ku, Chiba 2618502, Japan
来源：
JOURNAL OF PHARMACOLOGICAL SCIENCES | 2010年 / 112卷 / 02期
关键词：
transporter; enamel; mineralization; channel; SLC8 gene family; DENTAL ENAMEL CELLS; NA+/CA2+ EXCHANGE; ENDOPLASMIC-RETICULUM; VENTRICULAR MYOCYTES; GENE-EXPRESSION; SECRETORY STAGE; UP-REGULATION; IN-VITRO; MATURATION; INHIBITION;
D O I：
10.1254/jphs.09267FP
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Although the central role of ameloblasts in synthesis and resorption of enamel matrix proteins during amelogenesis is well documented, the Ca2+-transport/extrusing mechanism remains to be fully elucidated. To clarify Ca2+-transport in rat ameloblasts, we investigated expression and localization of Na+-Ca2+ exchanger (NCX) isoforms and the functional characteristics of their ion transporting/pharmacological properties RT-PCT and immunohistochemical analyses revealed expression of NCX1 and NCX3 in ameloblasts, localized in the apical membrane. In patch-clamp recordings, Ca2+ efflux by Na+-Ca2+ exchange showed dependence on external Na+ Ca2+ influx by Na+-Ca2+ exchange, measured by fura-2 fluorescence, showed dependence on extracellular Ca2+ concentration, and it was blocked by NCX inhibitors KB-R7943, SEA0400, and SN-6. These results showed significant expression of NCX1 and NCX3 in ameloblasts, indicating their involvement in the directional Ca2+ extrusion pathway from cells to the enamel mineralizing front.
引用
收藏
页码：223 / 230
页数：8
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