No differences in the body fat after violating core bioelectrical impedance measurement assumptions

ObjectiveIt is unclear to what degree acutely violating bioelectrical impedance analysis (BIA) measurement assumptions will alter the predicted percent fat mass (%FM) and whether this differs by sex or body mass index (BMI).Methods%FM was assessed under control, dehydration, exercise, water, food intake and non-voided bladder conditions with three BIA devices (Tanita: BC-418, TBF-314, & Omron HBF-306CN) for men (n =23, age: 24.05.2years old) and women (n =17, age: 22.53.4years old) separately.Results For all BIA devices, there were no differences in the %FM values between the control and the other conditions in men or women (-1.9 to 0.4%, p>0.05). Across the three devices and five conditions, 97% of %FM tests returned values within 5% of control (2 tests), and 86% of tests were within 2% of control despite violating an assumption. The errors were greatest with dehydration and women were more likely to have a %FM difference greater than 2% than men with dehydration using the hand-to-foot device (Tanita TBF-314: 59% versus 9%). There were no differences in %FM between control and the conditions when examined by BMI (overweight/obesity: -2.8 to 0.1% and normal weight: -1.7 to 0.5%; BMI*trial, p=0.99).Conclusion %FM estimates were similar despite acutely violating the preliminary measurement BIA assumptions across a range of different BMIs. The minor variations in %FM are smaller than what would be expected with day-to-day variability or weight loss intervention but may be larger in women than men.