Agrobacterium tumefaciens-mediated genetic transformation and plant regeneration from a complex tetraploid hybrid citrus rootstock

被引：16
作者：
Dutt, M. [1 ]
Madhavaraj, J. [1 ]
Grosser, J. W. [1 ]
机构：
[1] Univ Florida, Ctr Citrus Res & Educ, IFAS, Dept Hort Sci, Lake Alfred, FL 33850 USA
来源：
SCIENTIA HORTICULTURAE | 2010年 / 123卷 / 04期
关键词：
Agrobacterium tumefaciens; Bifunctional gene; Citrus; EGFP; Genetic transformation; Tetrazyg; Tetraploid; SOMATIC HYBRIDIZATION; SWEET ORANGE; PROTOPLAST FUSION; EXPRESSION;
D O I：
10.1016/j.scienta.2009.10.010
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Agrobacterium-mediated genetic transformation of a tetraploid "tetrazyg" citrus rootstock selection 'Orange #16' [Nova mandarin (Citrus reticulata Blanco) + Hirado Buntan pummelo (Citrus grandis L. Osbeck)] x [Cleopatra mandarin (C. reticulata Blanco) + Argentine trifoliate orange (Poncirus trifoliata (L.) Raf.)] was performed. juvenile epicotyl segments were transformed with a construct containing a bifunctional egfp-nptII fusion gene under the control of an enhanced double CaMV 35S promoter. Our protocol resulted in a reasonable transformation efficiency of 18%. Stable integration of the transgene was confirmed by Visual observation of EGFP expression, PCR and Southern blot hybridization. The purpose of this work was to investigate the amenability of novel citrus rootstock germplasm being developed for improved tree size control, soil adaptation, and disease resistance, to existing transformation technologies. Seed trees of such transgenic tetraploids also have potential as trap plants containing potent insecticidal transgenes, due to their inedible fruit and inherent crossing barriers with conventional commercial diploid scion cultivars, and could be planted around producing citrus groves. (C) 2009 Elsevier B.V. All rights reserved.
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收藏
页码：454 / 458
页数：5
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