Death inducer-obliterator 1 triggers apoptosis after nuclear translocation and caspase upregulation

被引：36

作者：

García-Domingo, D ^{[1
]}

Ramírez, D ^{[1
]}

de Buitrago, GG ^{[1
]}

Martínez, C ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Dept Immunol & Oncol, Ctr Nacl Biotecnol, CSIC, E-28049 Madrid, Spain

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2003年 / 23卷 / 09期

关键词：

D O I：

10.1128/MCB.23.9.3216-3225.2003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Death inducer-obliterator I (DIO-1) is a gene that is upregulated early in apoptosis. Here we report that in healthy cells, the DIO-1 gene product was located in the cytoplasm, where it formed oligomers. After interleukin-3 starvation or c-Myc-induced apoptosis in serum-free conditions, DIO-1 translocated to the nucleus, where it upregulated caspase levels and activity. A nuclear localization signal deletion mutant (DIO-1DeltaNLS) was unable to translocate to the nuclear compartment in the absence of interleukin-3 and failed to upregulate procaspase levels or trigger cell death. In addition, cells stably expressing DIO-1DeltaNLS were protected from apoptosis induced by interleukin-3 withdrawal. These results indicate that DIO-1 has a relevant role in regulating the early stages of cell death.

引用

页码：3216 / 3225

页数：10

共 42 条

[1] Human ICE/CED-3 protease nomenclature [J].

Alnemri, ES ;

Livingston, DJ ;

Nicholson, DW ;

Salvesen, G ;

Thornberry, NA ;

Wong, WW ;

Yuan, JY .

CELL, 1996, 87 (02) :171-171

[2] Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB [J].

Bertin, J ;

Nir, WJ ;

Fischer, CM ;

Tayber, OV ;

Errada, PR ;

Grant, JR ;

Keilty, JJ ;

Gosselin, ML ;

Robison, KE ;

Wong, GHW ;

Glucksmann, MA ;

DiStefano, PS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (19) :12955-12958

[3] Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death [J].

Boldin, MP ;

Goncharov, TM ;

Goltsev, YV ;

Wallach, D .

CELL, 1996, 85 (06) :803-815

[4] Biochemical pathways of caspase activation during apoptosis [J].

Budihardjo, I ;

Oliver, H ;

Lutter, M ;

Luo, X ;

Wang, XD .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1999, 15 :269-290

[5] Apaf-1/cytochrome c-independent and Smac-dependent induction of apoptosis in multiple myeloma (MM) cells [J].

Chauhan, D ;

Hideshima, T ;

Rosen, S ;

Reed, JC ;

Kharbanda, S ;

Anderson, KC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (27) :24453-24456

[6] Interferon γ induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells [J].

Dai, CH ;

Krantz, SB .

BLOOD, 1999, 93 (10) :3309-3316

[7] Mammalian caspases: Structure, activation, substrates, and functions during apoptosis [J].

Earnshaw, WC ;

Martins, LM ;

Kaufmann, SH .

ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 :383-424

[8] MECHANISMS AND FUNCTIONS OF CELL-DEATH [J].

ELLIS, RE ;

YUAN, JY ;

HORVITZ, HR .

ANNUAL REVIEW OF CELL BIOLOGY, 1991, 7 :663-698

[9] DIO-1 is a gene involved in onset of apoptosis in vitro, whose misexpression disrupts limb development [J].

García-Domingo, D ;

Leonardo, E ;

Grandien, A ;

Martínez, P ;

Albar, JP ;

Izpisúa-Belmonte, JC ;

Martínez, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (14) :7992-7997

[10] Enforced dimerization of BAX results in its translocation, mitochondrial dysfunction and apoptosis [J].

Gross, A ;

Jockel, J ;

Wei, MC ;

Korsmeyer, SJ .

EMBO JOURNAL, 1998, 17 (14) :3878-3885

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