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Effects of High-Intensity Ultrasound Pretreatment on Structure, Properties, and Enzymolysis of Soy Protein Isolate
被引:61
|作者:
Zhao, Fei
[1
,2
]
Liu, Xuemei
[3
]
Ding, Xiuzhen
[1
,2
]
Dong, Haizhou
[1
,2
]
Wang, Wentao
[1
,2
,4
]
机构:
[1] Shandong Agr Univ, Dept Food Sci & Engn, Tai An 271018, Shandong, Peoples R China
[2] Engn & Technol Ctr Grain Proc Shandong Prov, Tai An 271018, Shandong, Peoples R China
[3] All China Federat Supply & Mkt Cooperat, Jinan Fruit Res Inst, Jinan 250014, Shandong, Peoples R China
[4] Shandong Acad Sci, Qilu Univ Technol, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Shandong, Peoples R China
来源:
关键词:
high-intensity ultrasonication;
soybean protein isolates;
near-infrared spectra;
zeta-potential;
dynamic and static light scattering;
antioxidant activity;
HIGH-PRESSURE HOMOGENIZATION;
FUNCTIONAL-PROPERTIES;
PHYSICAL-PROPERTIES;
SECONDARY STRUCTURE;
AQUEOUS-SOLUTIONS;
BETA-CONGLYCININ;
HYDROLYSIS;
SIZE;
GLYCININ;
PEPSIN;
D O I:
10.3390/molecules24203637
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The objective of this study was to investigate the effects of different high-intensity ultrasonication (HIU) pretreatment on the structure and properties of soybean protein isolate (SPI) as well as enzymatic hydrolysis of SPI by bromelain and antioxidant activity of hydrolysates. The HIU-treated SPI fractions showed a decrease in the proportion of alpha -helices and beta -turns and an increase in the content of beta -sheets and random coils based on Fourier-transform infrared spectroscopy. Near-infrared spectra and fluorescence spectra analyses provided support for the changes in secondary and tertiary structures of SPI after ultrasound treatment. The particle size of SPI decreased from 217.20 nm to 141.23 nm and the absolute zeta potential increased. Scanning electron microscopy showed that HIU treatment changed apparent morphology. Dynamic and static light scattering of ultrasonicated samples showed that SPI structure had changed from hard-sphere to hollow-sphere or polydisperse and monodisperse gaussian coils. HIU pretreatment significantly increased the hydroxyl-radical scavenging and the degree of hydrolysis of the SPI hydrolysates.
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页数:17
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