Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer

被引：54

作者：

Kuettel, Claudia ^{[1
]}

Nascimento, Elisabete

Demierre, Nicolas

Silva, Tiago

Braschler, Thomas

Renaud, Philippe

Oliva, Abel G.

机构：

[1] ETH, Nanotechnol Grp, CH-8092 Zurich, Switzerland

[2] Ecole Polytech Fed Lausanne, Lab Microsyst, CH-1015 Lausanne, Switzerland

[3] Univ Nova Lisboa, Inst Tecnol Quim & Biol, Inst Biol Expt & Tecnol, P-2781 Oeiras, Portugal

来源：

ACTA TROPICA | 2007年 / 102卷 / 01期

关键词：

label-free; Babesin bovis; impedance spectroscopy; dielectrophoretic parasite detection; infected erythrocytes; microfabricated flow cytometer;

D O I：

10.1016/j.actatropica.2007.03.002

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The cellular modifications caused by the intracellular parasite result in a shift in impedance which can be measured dielectrically. Thus, a rapid cell-by-cell detection with microliter amounts of reagents is possible. Unlike other diagnostic tests, this method does not depend on extensive sample pre-treatment or expensive chemicals and equipment. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：63 / 68

页数：6

共 24 条

[1] Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum:: different logics achieve the same goal [J].