In vivo and in vitro tissue-specific expression of green fluorescent protein using the cre-lox system in mouse embryonic stem cells

被引：8
作者：
Schindehütte, J
Fukumitsu, H
Collombat, P
Griesel, G
Brink, C
Baier, PC
Capecchi, MR
Mansouri, A
机构：
[1] Univ Gottingen, Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany
[2] Univ Gottingen, Dept Clin Neurophysiol, D-3400 Gottingen, Germany
[3] Gifu Pharmaceut Univ, Mol Biol Lab, Gifu, Japan
[4] Univ Utah, Sch Med, Howard Hughes Med Inst, Salt Lake City, UT USA
[5] Univ Utah, Dept Human Genet, Salt Lake City, UT USA
来源：
STEM CELLS | 2005年 / 23卷 / 01期
关键词：
conditional green fluorescent protein expression; embryonic stem cells; transplantation; mouse;
D O I：
10.1634/stemcells.2004-0163
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Embryonic stem cells (ES) are pluripotent and may therefore serve as a source for the generation of specific cell types required for future therapies based on cell replacement. The isolation of defined cell populations from a certain lineage or tissue is a prerequisite for the analysis of the potential of such ES-derived cells in animal transplantation studies. Here, using the Cre/loxP system, we report the generation of murine ES cells conditionally expressing the hrGFP gene at the cell surface. Such ES cells can be differentiated in vitro into neurons displaying GFP activity in neurites. Transgenic mice derived from these ES cells permit the targeting of GFP-expression to specific tissues and provide material from the three germ layers suitable for molecular and biochemical analysis.
引用
收藏
页码：10 / 15
页数：6
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