Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel α1 subunits in the dorsal horn of spinal cord in mice

被引：59
作者：
Murakami, M
Nakagawasai, O
Suzuki, T
Mobarakeh, II
Sakurada, Y
Murata, A
Yamadera, F
Miyoshi, I
Yanai, K
Tan, K
Sasano, H
Tadano, T
Iijima, T
机构：
[1] Akita Univ, Sch Med, Dept Pharmacol, Akita 0108543, Japan
[2] Tohoku Pharaceut Univ, Dept Pharmacol, Sendai, Miyagi, Japan
[3] Tohoku Univ, Sch Med, Dept Pathol, Sendai, Miyagi, Japan
[4] Tohoku Univ, Sch Med, Dept Cellular Pharmacol, Sendai, Miyagi, Japan
[5] Tohoku Univ, Sch Med, Inst Anim Expt, Sendai, Miyagi, Japan
来源：
BRAIN RESEARCH | 2004年 / 1024卷 / 1-2期
关键词：
calcium channel; omega-agatoxin TK; SNX-482; nociception; toxin; mouse;
D O I：
10.1016/j.brainres.2004.07.066
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
To understand better which voltage-dependent calcium channels (VGCCs) are involved in nociceptive neurotransmission, we investigated the pharmacological properties and distribution of VGCCs in the mouse spinal cord. A behavioral assay revealed that intrathecal injections of omega-agatoxin TK, omega-agatoxin IVA, omega-conotoxin GVIA, and SNX-482, which block P/Q-, P/Q-, N-, and R-type calcium channels, respectively, produced analgesic effects, while an L-type channel blocker had no such effect. An electrophysiological study demonstrated the presence of various types of VGCCs within dorsal root ganglion (DRG) neurons. Immunohistochemistry revealed distinct localization of P/Q-, N-, L-, and R-type calcium channel subunits to the dorsal horn of the spinal cord. The results of this study revealed the localization and functions of several calcium channels that are involved in nociceptive neurotransmission within the dorsal horn of the mouse spinal cord. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：122 / 129
页数：8
相关论文
共 24 条
[1]   International Union of Pharmacology. XL. Compendium of voltage-gated ion channels: Calcium channels [J].
Catterall, WA ;
Striessnig, J ;
Snutch, TP ;
Perez-Reyes, E .
PHARMACOLOGICAL REVIEWS, 2003, 55 (04) :579-581
[2]   Blockade of spinal N- and P-type, but not L-type, calcium channels inhibits the excitability of rat dorsal horn neurones produced by subcutaneous formalin inflammation [J].
Diaz, A ;
Dickenson, AH .
PAIN, 1997, 69 (1-2) :93-100
[3]  
ELLIOTT EM, 1995, J NEUROSCI, V15, P6433
[4]   NEUROANATOMICAL DISTRIBUTION OF RECEPTORS FOR A NOVEL VOLTAGE-SENSITIVE CALCIUM-CHANNEL ANTAGONIST, SNX-230 (OMEGA-CONOPEPTIDE-MVIIC) [J].
GOHIL, K ;
BELL, JR ;
RAMACHANDRAN, J ;
MILJANICH, GP .
BRAIN RESEARCH, 1994, 653 (1-2) :258-266
[5]  
HILLYARD DR, 1992, NEURON, V9, P67
[6]   Ablation of P/Q-type Ca2+ channel currents, altered synaptic transmission, and progressive ataxia in mice lacking the α1A-subunit [J].
Jun, K ;
Piedras-Renteria, ES ;
Smith, SM ;
Wheeler, DB ;
Lee, SB ;
Lee, TG ;
Chin, HM ;
Adams, ME ;
Scheller, RH ;
Tsien, RW ;
Shin, HS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :15245-15250
[7]  
KANDEL E, 2000, PAIN ANALGESIA PRINC, P385
[8]  
MALMBERG AB, 1994, J NEUROSCI, V14, P4882
[9]   OMEGA-CONOTOXIN - DIRECT AND PERSISTENT BLOCKADE OF SPECIFIC TYPES OF CALCIUM CHANNELS IN NEURONS BUT NOT MUSCLE [J].
MCCLESKEY, EW ;
FOX, AP ;
FELDMAN, DH ;
CRUZ, LJ ;
OLIVERA, BM ;
TSIEN, RW ;
YOSHIKAMI, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) :4327-4331
[10]   P-TYPE CALCIUM CHANNELS IN RAT CENTRAL AND PERIPHERAL NEURONS [J].
MINTZ, IM ;
ADAMS, ME ;
BEAN, BP .
NEURON, 1992, 9 (01) :85-95
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