Dual-functional probe based on rhodamine for sequential Cu2+ and ATP detection in vivo

被引：34

作者：

Jin, Xilang ^{[1
]}

Gao, Jingkai ^{[2
]}

Xie, Pu ^{[1
]}

Yu, Mengchen ^{[3
]}

Wang, Ting ^{[1
]}

Zhou, Hongwei ^{[1
]}

Ma, Aijie ^{[1
]}

Wang, Qian ^{[1
]}

Leng, Xin ^{[4
]}

Zhang, Xianghan ^{[2
]}

机构：

[1] Xian Technol Univ, Sch Mat & Chem Engn, Xian 710032, Shaanxi, Peoples R China

[2] Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China

[3] Xian Technol Univ, State & Local Joint Engn Lab Adv Network & Monito, Xian 710032, Shaanxi, Peoples R China

[4] Northwest Univ, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710069, Shaanxi, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2018年 / 204卷

基金：

中国国家自然科学基金;

关键词：

Rhodamine; Fluorescence; ATP; Cu2+; Bioimaging; FLUORESCENT-PROBE; LIVING CELLS; LIVE CELLS; MITOCHONDRIA; RECOGNITION; CHEMOSENSOR; DERIVATIVES; FLUOROPHORE; AMINO; HG2+;

D O I：

10.1016/j.saa.2018.06.094

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A rhodamine-based fluorescent probe for Cu2+ and ATP has been designed. The fluorescence intensity/absorbance was significantly enhanced upon the addition of Cu2+ owning to the opening of the Spiro-ring of rhodamine, which quickly returned to the original level due to the reconstruction of the probe by the reacting with ATP. Cu2+/ATP-induced fluorescent intensity/aborbance changes showed a good linear relationship with the concentration of Cu2+/ATP in the range of 2-20 mu M/0-10 mu M with a detection limit of 0.1 mu M/1.0 mu M. The proposed method is simple in design and fast in operation, and is suitable for the reversible monitoring of Cu2+ and ATP in bioanalytical applications. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：657 / 664

页数：8

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