Ribosome-mediated incorporation of fluorescent amino acids into peptides in vitro

被引：15

作者：

Lee, Joongoo ^{[1
,2
]}

Schwarz, Kevin J. ^{[3
]}

Yu, Hao ^{[4
,5
]}

Kruger, Antje ^{[1
,2
]}

Anslyn, Eric, V ^{[6
]}

Ellington, Andrew D. ^{[7
]}

Moore, Jeffrey S. ^{[3
,8
]}

Jewett, Michael C. ^{[1
,2
]}

机构：

[1] Northwestern Univ, Dept Chem & Biol Engn, Evanston, IL 60208 USA

[2] Northwestern Univ, Ctr Synthet Biol, Evanston, IL 60208 USA

[3] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Chem Engn, Urbana, IL 61801 USA

[5] Univ Illinois, Dept Biomol Engn, Urbana, IL 61801 USA

[6] Univ Texas Austin, Dept Chem & Biochem, Austin, TX 78712 USA

[7] Univ Texas Austin, Dept Chem & Biochem, Inst Cellular & Mol Biol, Austin, TX 78712 USA

[8] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA

来源：

CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 21期

关键词：

Fluorescence; -; RNA;

D O I：

10.1039/d0cc07740b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosome in vitro through genetic code reprogramming.

引用

页码：2661 / 2664

页数：4

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