Oxidation at a sol-gel composite electrode doped with a dirhodium-substituted polyoxometalate for amperometric detection of peptides separated by HPLC

被引：15

作者：

Cox, JA ^{[1
]}

Holmstrom, SD ^{[1
]}

Tess, ME ^{[1
]}

机构：

[1] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA

来源：

TALANTA | 2000年 / 52卷 / 06期

关键词：

electrocatalysis; peptides; transition-metal-substituted polyoxometalate; sol-gel chemistry; carbon-ceramic composite;

D O I：

10.1016/S0039-9140(00)00477-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Oxidation of a variety of compounds, including methionine (Met), using a complex formed between dirhodium(II) acetate and the lacunary form of phosphotungstic acid as the catalyst is effective over a wide range of conditions, including pH 2-10. Thus, amperometric detection at a composite in which this complex is immobilized in a sol-gel material does not place restrictions on selection of conditions for separations by reverse-phase HPLC. A demonstration of this point is shown by a study of Met, Met-Phe, Phe-Met, Met-Met, and Gly-Met-Gly (Phe, phenylalanine; Gly, glycine). Using a 0.05 M phosphate buffer at pH 6.7, a C18 column, and a flow rate of 1 ml min(-1) capacity factors for Met, Gly-Met-Gly, Met-Met, and Phe-Met were 1.4, 2.1, 5.6, and 34, respectively. Phe-Met and Met-Phe co-eluted. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：1081 / 1086

页数：6

共 23 条

[1] A NEW GENERAL STRUCTURAL CATEGORY OF HETEROPOLYELECTROLYTES . UNUSUAL MAGNETIC AND THERMAL CONTRACTION PHENOMENA [J].