Genomic Definition of Hypervirulent and Multidrug-Resistant Klebsiella pneumoniae Clonal Groups

被引：379

作者：

Bialek-Davenet, Suzanne ^{[1
,2
,3
]}

Criscuolo, Alexis ^{[1
,2
]}

Ailloud, Florent ^{[1
]}

Passet, Virginie ^{[1
,2
]}

Jones, Louis ^{[1
]}

Delannoy-Vieillard, Anne-Sophie ^{[1
]}

Garin, Benoit ^{[4
]}

Le Hello, Simon ^{[1
]}

Arlet, Guillaume ^{[5
,6
,7
]}

Nicolas-Chanoine, Marie-Helene ^{[3
,6
,8
]}

Decre, Dominique ^{[5
,6
,7
]}

Brisse, Sylvain ^{[1
,2
]}

机构：

[1] Inst Pasteur, F-75724 Paris, France

[2] CNRS, Paris, France

[3] Hop Beaujon, Clichy, France

[4] Inst Pasteur, Antananarivo, Madagascar

[5] Univ Paris 04, Paris, France

[6] INSERM, Paris, France

[7] Hop Est Parisien, Paris, France

[8] Univ Paris Diderot, Fac Med, Paris, France

来源：

EMERGING INFECTIOUS DISEASES | 2014年 / 20卷 / 11期

关键词：

CHROMOSOMAL BETA-LACTAMASE; NOSOCOMIAL PATHOGENS; LIVER-ABSCESS; EFFLUX PUMP; STRAINS; K1; OLAQUINDOX; GENE;

D O I：

10.3201/eid2011.140206

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined. We aimed to identify K. pneumoniae CGs on the basis of genonne-wide sequence variation and to provide a simple bioinformatics tool to extract virulence and resistance gene data from genomic data. We sequenced 48 K. pneumoniae isolates, mostly of serotypes K1 and K2, and compared the genomes with 119 publicly available genomes. A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed hypervirulent and multidrug-resistant CGs. In addition, we created a freely accessible database, BIGSdb-Kp, to enable rapid extraction of medically and epidemiologically relevant information from genomic sequences of K. pneumoniae. Although drug-resistant and virulent K. pneumoniae populations were largely nonoverlapping, isolates with combined virulence and resistance features were detected.

引用

页码：1812 / 1820

页数：9

共 40 条

[1] Comparative Population Analysis of Klebsiella pneumoniae Strains with Extended-Spectrum β-Lactamases Colonizing Patients in Rehabilitation Centers in Four Countries [J].

Baraniak, A. ;

Izdebski, R. ;

Fiett, J. ;

Sadowy, E. ;

Adler, A. ;

Kazma, M. ;

Salomon, J. ;

Lawrence, C. ;

Rossini, A. ;

Salvia, A. ;

Vidal Samso, J. ;

Fierro, J. ;

Paul, M. ;

Lerman, Y. ;

Malhotra-Kumar, S. ;

Lammens, C. ;

Goossens, H. ;

Hryniewicz, W. ;

Brun-Buisson, C. ;

Carmeli, Y. ;

Gniadkowski, M. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (04) :1992-1997

[2] Microbiological and clinical characteristics of bacteraemia caused by the hypermucoviscosity phenotype of Klebsiella pneumoniae in Korea [J].

Bialek-Davenet, Suzanne ;

Nicolas-Chanoine, Marie-Helene ;

Decre, Dominique ;

Brisse, Sylvain .

EPIDEMIOLOGY AND INFECTION, 2013, 141 (01) :188-188

[3] Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258 [J].

Breurec, S. ;

Guessennd, N. ;

Timinouni, M. ;

Le, T. A. H. ;

Cao, V. ;

Ngandjio, A. ;

Randrianirina, F. ;

Thiberge, J. M. ;

Kinana, A. ;

Dufougeray, A. ;

Perrier-Gros-Claude, J. D. ;

Boisier, P. ;

Garin, B. ;

Brisse, S. .

CLINICAL MICROBIOLOGY AND INFECTION, 2013, 19 (04) :349-355

[4] Virulent Clones of Klebsiella pneumoniae: Identification and Evolutionary Scenario Based on Genomic and Phenotypic Characterization [J].

Brisse, Sylvain ;

Fevre, Cindy ;

Passet, Virginie ;

Issenhuth-Jeanjean, Sylvie ;

Tournebize, Regis ;

Diancourt, Laure ;

Grimont, Patrick .

PLOS ONE, 2009, 4 (03)

[5] Whole-Genome Draft Sequences of Three Multidrug-Resistant Klebsiella pneumoniae Strains Available from the American Type Culture Collection [J].

Broberg, Christopher A. ;

Palacios, Michelle ;

Miller, Virginia L. .

GENOME ANNOUNCEMENTS, 2013, 1 (03)

[6] Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43 [J].

Chen, YT ;

Chang, HY ;

Lai, YC ;

Pan, CC ;

Tsai, SF ;

Peng, HL .

GENE, 2004, 337 :189-198

[7] Emerging Severe and Fatal Infections Due to Klebsiella pneumoniae in Two University Hospitals in France [J].

Decre, Dominique ;

Verdet, Charlotte ;

Emirian, Aurelie ;

Le Gourrierec, Thibault ;

Petit, Jean-Claude ;

Offenstadt, Georges ;

Maury, Eric ;

Brisse, Sylvain ;

Arlet, Guillaume .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (08) :3012-3014

[8] Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates [J].

Diancourt, L ;

Passet, V ;

Verhoef, J ;

Grimont, PAD ;

Brisse, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) :4178-4182

[9] Plasmid Content of a Clinically Relevant Klebsiella pneumoniae Clone from the Czech Republic Producing CTX-M-15 and QnrB1 [J].

Dolejska, Monika ;

Villa, Laura ;

Dobiasova, Hana ;

Fortini, Daniela ;

Feudi, Claudia ;

Carattoli, Alessandra .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (02) :1073-1076

[10] Klebsiella pneumoniae genotype K1:: an emerging pathogen that causes septic ocular or central nervous system complications from pyogenic liver abscess [J].

Fang, Chi-Tai ;

Lai, Shau-Yan ;

Yi, Wen-Ching ;

Hsueh, Po-Ren ;

Liu, Kao-Lang ;

Chang, Shan-Chwen .

CLINICAL INFECTIOUS DISEASES, 2007, 45 (03) :284-293

← 1 2 3 4 →