Role of cyclic alkyl group in conformational instability of tannase

被引：5

作者：

Nie, Guangjun ^{[1
,2
]}

Zhao, Rui ^{[1
]}

Sun, Wuyue ^{[1
]}

Gao, Yu ^{[1
]}

Zhu, Xiangxiang ^{[1
]}

Zheng, Zhiming ^{[2
]}

Yue, Wenjin ^{[1
]}

机构：

[1] Anhui Polytech Univ, Coll Biochem Engn, Wuhu 241000, Peoples R China

[2] Chinese Acad Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2016年 / 128卷

关键词：

Biocatalysis; FT-IR; Conformational stability; Tannase; Cyclic structure; ANTIOXIDANT PROPYL GALLATE; ORGANIC-SOLVENTS; ASPERGILLUS-NIGER; BIOCATALYSIS; ACTIVATION; ENZYME;

D O I：

10.1016/j.molcatb.2016.03.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conformational stability of enzyme has a crucial effect on its catalytic performance. The effects of six organic solvents with different structures on the conformational stability of tannase were studied using Fourier transform infrared spectroscopy in this work. This results indicated that the cyclic structure of organic solvent plays a negative role in the conformational stability of tannase. The alkyl group of organic solvent has an interaction with the groups of oxygen and nitrogen of tannase, and the interaction goes against the conformational stability. The findings potentially provide a deep insight into the relationship between the biocatalytic activity and conformational stability of enzymes and push the study on the interaction of enzyme with organic solvent. (C) 2016 Published by Elsevier B.V.

引用

页码：78 / 81

页数：4

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