Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs

被引：50
|
作者：
Ravula, Thirupathi [1 ,2 ]
Hardin, Nathaniel Z. [1 ,2 ]
Bai, Jia [1 ,2 ]
Im, Sang-Choul [3 ,4 ]
Waskell, Lucy [3 ,4 ]
Ramamoorthy, Ayyalusamy [1 ,2 ]
机构：
[1] Univ Michigan, Biophys, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Anesthesiol, Ann Arbor, MI 48105 USA
[4] VA Med Ctr, Ann Arbor, MI 48105 USA
来源：
CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 69期
关键词：
MALEIC ACID COPOLYMER; PHOSPHOLIPID-BILAYER NANODISCS; DETERGENT-FREE ISOLATION; NMR-SPECTROSCOPY; LIPID-BILAYER; CYTOCHROME-P450; 3A4; SOLUBILIZATION; B(5); NANOPARTICLES; INSIGHTS;
D O I：
10.1039/c8cc04184a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we demonstrate the functional reconstitution of a large soluble-domain containing positively-charged approximate to 57 kDa cytochrome-P450 and negatively-charged approximate to 16 kDa cytochrome-b5 in lipid-nanodiscs, and the role of the polymer charge for high-resolution studies on membrane proteins.
引用
收藏
页码：9615 / 9618
页数：4
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