Anticancer Activities of Antimicrobial BmKn2 Peptides Against Oral and Colon Cancer Cells

被引：24

作者：

Arpornsuwan, Teerakul ^{[1
]}

Sriwai, Wimolpak ^{[1
]}

Jaresitthikunchai, Janthima ^{[2
]}

Phaonakrop, Narumon ^{[2
]}

Sritanaudomchai, Hathaitip ^{[3
]}

Roytrakul, Sittiruk ^{[2
]}

机构：

[1] Thammasat Univ, Fac Allied Hlth Sci, Dept Med Technol, Pathum Thani, Thailand

[2] Natl Ctr Genet Engn & Biotechnol, Genome Inst, Pathum Thani, Thailand

[3] Mahidol Univ, Fac Dent, Bangkok 10700, Thailand

来源：

INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS | 2014年 / 20卷 / 04期

关键词：

Antimicrobial peptide; Antitumor peptide; SW620; cells; HSC4; Dental pulp stem cells; IN-VITRO; HYDROPHOBICITY; IDENTIFICATION; ANTIBACTERIAL; PREDICTION; PROTEINS; CHARGE; DEATH; VENOM;

D O I：

10.1007/s10989-014-9417-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is considerable current interest in developing antimicrobial and anticancer agents with a new mode of action. The antimicrobial peptides are regarded as a potential solution for treating cancer cells. The antimicrobial effect of 6 synthetic peptides against 7 bacterial species was evaluated. The result showed that IsCT, BmKn2 and BMAP-28 exhibited broad range of action against Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538, methicillin resistant S. aureus DMST 20651, Staphylococcus epidermidis ATCC 12228, Acinetobacter baumanii ATCC 19066, Escherichia coli ATCC 25922 and Salmonella typhi DMST 562 at minimal inhibitory concentrations (MIC) of 2.97-24.28 mu M. Neither AMP induced significant hemolysis, or showed cytotoxic on dental pulp stem cells and smooth muscle cells at their MICs. In addition, BmKn2 inhibited growth of human oral squamous carcinoma HSC4 cells and human colon cancer SW620 cells with IC50 of 17.26 and 40 A mu M, respectively. Taken together, BmKn2 peptide from scorpion venom may offer a novel therapeutic strategy for development of cationic antimicrobial and anticancer peptides as potential new therapeutic agents.

引用

页码：501 / 509

页数：9

共 46 条

[1] Tumor cell-selective antiproliferative effect of the extract from Morinda citrifolia fruits [J].

Arpornsuwan, Teerakul ;

Punjanon, Tadsanee .

PHYTOTHERAPY RESEARCH, 2006, 20 (06) :515-517

[2] Anticancer mechanisms of action of two small amphipathic β2,2-amino acid derivatives derived from antimicrobial peptides [J].

Ausbacher, Dominik ;

Svineng, Gunbjorg ;

Hansen, Terkel ;

Strom, Morten B. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2012, 1818 (11) :2917-2925

[3] In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs [J].

Benincasa, M ;

Skerlavaj, B ;

Gennaro, R ;

Pellegrini, A ;

Zanetti, M .

PEPTIDES, 2003, 24 (11) :1723-1731

[4] Antibacterial Activity and Mechanism of a Scorpion Venom Peptide Derivative In Vitro and In Vivo [J].

Cao, Luyang ;

Dai, Chao ;

Li, Zhongjie ;

Fan, Zheng ;

Song, Yu ;

Wu, Yingliang ;

Cao, Zhijian ;

Li, Wenxin .

PLOS ONE, 2012, 7 (07)

[5] PR-39, a syndecan-inducing antimicrobial peptide, binds and affects p130Cas [J].

Chan, YR ;

Gallo, RL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (44) :28978-28985

[6] Role of peptide hydrophobicity in the mechanism of action of α-helical antimicrobial peptides [J].

Chen, Yuxin ;

Guarnieri, Michael T. ;

Vasil, Adriana I. ;

Vasil, Michael L. ;

Mant, Colin T. ;

Hodges, Robert S. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (04) :1398-1406

[7] ERYTHROCYTE-MEMBRANE LIPID REORGANIZATION DURING THE SICKLING PROCESS [J].

CHIU, D ;

LUBIN, B ;

SHOHET, SB .

BRITISH JOURNAL OF HAEMATOLOGY, 1979, 41 (02) :223-234

[8] Mucroporin, the First Cationic Host Defense Peptide from the Venom of Lychas mucronatus [J].

Dai, Chao ;

Ma, Yibao ;

Zhao, Zhenhuan ;

Zhao, Ruiming ;

Wang, Qian ;

Wu, Yingliang ;

Cao, Zhijian ;

Li, Wenxin .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (11) :3967-3972

[9] Optimization of the antimicrobial activity of magainin peptides by modification of charge [J].

Dathe, M ;

Nikolenko, H ;

Meyer, J ;

Beyermann, M ;

Bienert, M .

FEBS LETTERS, 2001, 501 (2-3) :146-150

[10] THE ROLE OF CHARGE AND HYDROPHOBICITY IN PEPTIDE LIPID INTERACTION - A COMPARATIVE-STUDY BASED ON TRYPTOPHAN FLUORESCENCE MEASUREMENTS COMBINED WITH THE USE OF AQUEOUS AND HYDROPHOBIC QUENCHERS [J].

DEKROON, AIP ;

SOEKARJO, MW ;

DEGIER, J ;

DEKRUIJFF, B .

BIOCHEMISTRY, 1990, 29 (36) :8229-8240

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