Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo

被引：23
|
作者：
Tan, LP
Lue, RYP
Chen, GYJ
Yao, SQ
机构：
[1] Natl Univ Singapore, Dept Sci Biol, Singapore 117543, Singapore
[2] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 24期
关键词：
intein; protein biotinylation; protein array; proteomics;
D O I：
10.1016/j.bmcl.2004.09.083
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6067 / 6070
页数：4
相关论文
共 50 条
  • [1] Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo
    Charalambous A.
    Andreou M.
    Skourides P.A.
    Journal of Nanobiotechnology, 7 (1)
  • [2] Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing
    Girish, A
    Sun, HY
    Yeo, DSY
    Chen, GYJ
    Chua, TK
    Yao, SQ
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2005, 15 (10) : 2447 - 2451
  • [3] Intein-mediated biotinylation of proteins and its application in a protein microarray
    Lesaicherre, ML
    Lue, RYP
    Chen, GYJ
    Zhu, Q
    Yao, SQ
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (30) : 8768 - 8769
  • [4] Intein-mediated, in vitro and in vivo protein modifications with small molecules
    Tan, LP
    Yao, SQ
    PROTEIN AND PEPTIDE LETTERS, 2005, 12 (08): : 769 - 775
  • [5] Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays
    Souvik Chattopadhaya
    Lay Pheng Tan
    Shao Q Yao
    Nature Protocols, 2006, 1 : 2386 - 2398
  • [6] Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems:: toward functional protein arrays
    Chattopadhaya, Souvik
    Tan, Lay Pheng
    Yao, Shao Q.
    NATURE PROTOCOLS, 2006, 1 (05) : 2386 - 2398
  • [7] An Intein-Mediated Site-Specific Click Conjugation Strategy for Improved Tumor Targeting of Nanoparticle Systems
    Elias, Drew R.
    Cheng, Zhiliang
    Tsourkas, Andrew
    SMALL, 2010, 6 (21) : 2460 - 2468
  • [8] Recent advances in in vivo applications of intein-mediated protein splicing
    Natalya I Topilina
    Kenneth V Mills
    Mobile DNA, 5
  • [9] Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems
    Cui, Chunxian
    Zhao, Wentao
    Chen, Jianglei
    Wang, Jianjun
    Li, Qianqian
    PROTEIN EXPRESSION AND PURIFICATION, 2006, 50 (01) : 74 - 81
  • [10] Site-specific protein cleavage in vivo by an intein-derived protease
    Volkmann, Gerrit
    Volkmann, Verena
    Liu, Xiang-Qin
    FEBS LETTERS, 2012, 586 (01) : 79 - 84
12345