Relationships of Age and Sex with Cytokine Content and Distribution in Human Platelet Fibrin Gels

被引：10

作者：

Bai, Meng-Yi ^{[1
,2
]}

Chuang, Meng-Han ^{[1
]}

Lin, Ming-Fang ^{[3
,4
]}

Tang, Sung-Ling ^{[2
]}

Wong, Chin-Chean ^{[5
,6
]}

Chan, Wing P. ^{[3
,7
]}

机构：

[1] Natl Taiwan Univ Sci & Technol, Grad Inst Biomed Engn, Taipei 10607, Taiwan

[2] Natl Taiwan Univ Sci & Technol, Grad Inst Appl Sci & Technol, Biomed Engn Program, Taipei 10607, Taiwan

[3] Taipei Med Univ, Wan Fang Hosp, Dept Radiol, Taipei 11617, Taiwan

[4] Yuanpei Univ, Dept Med Imaging & Radiol Technol, Hsinchu 30015, Taiwan

[5] Taipei Med Univ, Coll Med, Sch Med, Dept Orthoped, Taipei 11031, Taiwan

[6] Taipei Med Univ, Shuang Ho Hosp, Dept Orthoped, New Taipei 23561, Taiwan

[7] Taipei Med Univ, Coll Med, Sch Med, Dept Radiol, Taipei 110, Taiwan

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

RICH PLASMA PRP; GROWTH-FACTOR-I; REGENERATIVE MEDICINE;

D O I：

10.1038/s41598-018-28376-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We aimed to determine relationships between age and sex with cytokine content and distribution in human platelet-rich fibrin (PRF) gel. Rabbit PRF was harvested from whole blood (n = 6). Human PRF was collected from 36 healthy volunteers (1: 1 men: women) without systemic diseases and not current undergoing medical treatment. Histological analysis and optical microscopy were used to assess the three-dimensional structure of the PRF network. Enzyme-linked immunosorbent assays, quantification of adenosine triphosphate, and bioluminescence imaging of PRF sections were used to assess cytokine and entrapped platelet distribution. Three-dimensional structures of fibrin networks revealed concentration gradients of the platelet-derived growth factor beta beta homodimer and the transforming growth factor-beta 1. Histological analysis of PRF sections (from the red blood cell end to the plasma end of a clot) showed a gradual increase in average porosity, most prominently in PRF clots from young and middle-aged men and women, and a decrease in compactness along the longitudinal axis of the PRF gel. The end of the PRF gel closest to the red blood cell layer is the essence of the PRF clot, and the ability to generate platelets depends on sex and age in humans.

引用

页数：9

共 28 条

[1] Evaluation of growth hormone and insulin-like growth factor-1 in children during and after therapy for acute lymphoblastic leukemia [J].

Al-Azzawi, Mahmood A. ;

Elmadbouh, Ibrahim ;

Al-Azzawi, Thabit N. ;

Humood, Tariq A. ;

Ghanayem, Naglaa M. .

EGYPTIAN JOURNAL OF HAEMATOLOGY, 2014, 39 (04) :238-245

[2] Platelet-rich plasma for managing pain and inflammation in osteoarthritis [J].

Andia, Isabel ;

Maffulli, Nicola .

NATURE REVIEWS RHEUMATOLOGY, 2013, 9 (12) :721-730

[3] Three-dimensional structure and cytokine distribution of platelet-rich fibrin [J].

Bai, Meng-Yi ;

Wang, Ching-Wei ;

Wang, Jyun-Yi ;

Lin, Ming-Fang ;

Chan, Wing P. .

CLINICS, 2017, 72 (02) :116-124

[4] Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF): Surgical Adjuvants, Preparations for In Situ Regenerative Medicine and Tools for Tissue Engineering [J].

Bielecki, Tomasz ;

Ehrenfest, David M. Dohan .

CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2012, 13 (07) :1121-1130

[5] Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility [J].

Cruz-Orengo, Lillian ;

Daniels, Brian P. ;

Dorsey, Denise ;

Basak, Sarah Alison ;

Grajales-Reyes, Jose G. ;

McCandless, Erin E. ;

Piccio, Laura ;

Schmidt, Robert E. ;

Cross, Anne H. ;

Crosby, Seth D. ;

Klein, Robyn S. .

JOURNAL OF CLINICAL INVESTIGATION, 2014, 124 (06) :2571-2584

[6]

Dohan David M, 2006, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, V101, pe37, DOI 10.1016/j.tripleo.2005.07.008

[7] Gender and Age Differences in Growth Factor Concentrations From Platelet-Rich Plasma in Adults [J].

Evanson, J. Richard ;

Guyton, M. Kelly ;

Oliver, David L. ;

Hire, Justin M. ;

Topolski, Richard L. ;

Zumbrun, Steven D. ;

McPherson, James C. ;

Bojescul, John A. .

MILITARY MEDICINE, 2014, 179 (07) :799-805

[8] Platelet-rich Plasma and Platelet-rich fibrin in human cell culture [J].

Galing, Volker L. W. ;

Acil, Yahya ;

Springer, Ingo N. ;

Hubert, Nina ;

Wiltfang, Joerg .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2009, 108 (01) :48-55

[9] Platelet-Rich Plasma (PRP) for Acute Muscle Injury: A Systematic Review [J].

Hamid, Mohamad Shariff A. ;

Yusof, Ashril ;

Ali, Mohamed Razif Mohamed .

PLOS ONE, 2014, 9 (02)

[10] Female versus male biological identities of nanoparticles determine the interaction with immune cells in fish [J].

Hayashi, Yuya ;

Miclaus, Teodora ;

Murugadoss, Sivakumar ;

Takamiya, Masanari ;

Scavenius, Carsten ;

Kjaer-Sorensen, Kasper ;

Enghild, Jan J. ;

Straehle, Uwe ;

Oxvig, Claus ;

Weiss, Carsten ;

Sutherland, Duncan S. .

ENVIRONMENTAL SCIENCE-NANO, 2017, 4 (04) :895-906

← 1 2 3 →