Expression, localization and control of activin A release from human umbilical vein endothelial cells

被引：3

作者：

Borges, Lavinia E. ^{[1
]}

Bloise, Enrrico ^{[2
]}

Dela Cruz, Cynthia ^{[1
]}

Massai, Lauretta ^{[3
]}

Ciarmela, Pasquapina ^{[4
]}

Apa, Rosanna ^{[5
]}

Luisi, Stefano ^{[6
]}

Severi, Filiberto M. ^{[6
]}

Petraglia, Felice ^{[6
]}

Reis, Fernando M. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Obstet & Gynecol, Belo Horizonte, MG, Brazil

[2] Univ Fed Rio de Janeiro, Biophys Inst Carlos Chagas Filho, Lab Translat Endocrinol, Rio De Janeiro, Brazil

[3] Univ Siena, Dept Med Surg & Neurol Sci, I-53100 Siena, Italy

[4] Polytech Univ Marche, Dept Expt & Clin Med, Ancona, Italy

[5] Univ Cattolica Sacro Cuore, Inst Obstet & Gynaecol, Rome, Italy

[6] Univ Siena, Dept Mol & Dev Med Obstet & Gynecol, I-53100 Siena, Italy

来源：

GROWTH FACTORS | 2015年 / 33卷 / 04期

关键词：

Activin A; cytokines; endothelium; HUVEC; umbilical cord; urocortin; 2-SITE ENZYME-IMMUNOASSAY; DIFFERENTIAL EXPRESSION; INFLAMMATORY RESPONSE; SECRETION; PREECLAMPSIA; FOLLISTATIN; MYOMETRIUM; RECEPTORS; INHIBINS; PLACENTA;

D O I：

10.3109/08977194.2015.1071809

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Activin-A is a member of the TGF beta superfamily found in maternal and umbilical cord blood throughout gestation. We investigated whether human umbilical vein endothelial cells (HUVEC) express activin-A in vivo and tested the effects of vasoactive (endothelin-1), pro-inflammatory (interferon-gamma, interleukin-8) and anti-inflammatory (dexamethasone, urocortin) factors on activin-A release by isolated HUVEC in vitro. Activin beta A subunit protein and mRNA were strongly localized in the endothelial cells of umbilical veins and were also detectable in scattered cells of the cord connective tissue. Dimeric activin-A was detected in the HUVEC culture medium at picomolar concentrations. Activin-A release by HUVEC decreased after cell incubation with urocortin (p<0.01), whereas no effect was observed with interleukin-8, interferon-gamma, endothelin-1 or dexamethasone. In summary, activin-A is present in the human umbilical vein endothelium in vivo and is produced and released by isolated HUVEC. Activin-A secretion is inhibited in vitro by urocortin, a neuropeptide with predominantly anti-inflammatory action.

引用

页码：243 / 249

页数：7

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