EFFECT OF PC-3 PROSTATE CANCER CELL LINE SUPERNATANT ON APOPTOSIS IN MACROPHAGES

Particular types of cell death, for example apoptosis, play important role in metastatic processes. Apoptosis is programmed cell death, which is characterised by specific morphological changes. In this study we aimed on topic of affecting of supernatant from prostate cancer cell line PC-3 on a healthy cells of immune system, macrophages concretely. Peripheral blood monocytes were cultivated for 7 days to macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) and cultivated with supernatant for another 24 hours. Preparations were prepared and analysed by light microscopy. Macrophages with normal morphology show the largest rate in our experiment. In samples macrophages + supernatant there was an increase mainly in late stage of apoptosis. A lot of observed cells showed feature of rupture and spillage of cell contents into the microenvironment. In samples macrophages + supernatant + LPS there is a noticeable decline in the incidence of normal morphology compared to the control. This means that effect of cancer cells and their supernatants on macrophages can induce apoptosis on macrophages and thus prevent proper course of immune responses.