Plasmid pGS5 from the hyperthermophilic archaeon Archaeaglobus profundus is negatively supercoiled

被引：25

作者：

López-García, P

Forterre, P

Van der Oost, J

Erauso, G

机构：

[1] Univ Miguel Hernandez, Fac Med, Div Microbiol, Alacant 03550, Spain

[2] Univ Paris 11, Inst Genet & Microbiol, F-91405 Orsay, France

[3] Univ Bretagne Occidentale, Inst Univ Europeen Mer, F-29280 Plouzane, France

[4] Agr Univ Wageningen, Dept Biomol Sci, Microbiol Lab, NL-6703 CT Wageningen, Netherlands

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 17期

关键词：

D O I：

10.1128/JB.182.17.4998-5000.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present evidence that, in contrast to plasmids from other hyperthermophilic archaea, which are in the relaxed to positively supercoiled state, plasmid pGS5 (2.8 kb) from Archaeoglobus profundus is negatively supercoiled. This might be due to the presence of a gyrase introducing negative supercoils, since gyrase genes are present in the genome of its close relative A. fulgidus, and suggests that gyrase activity predominates over reverse gyrase whenever the two topoisomerases coexist in cells.

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页码：4998 / 5000

页数：3

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