Inositol trisphosphate and cyclic ADP-ribose-mediated release of Ca2+ from single isolated pancreatic zymogen granules

被引:204
作者
Gerasimenko, OV [1 ]
Gerasimenko, JV [1 ]
Belan, PV [1 ]
Petersen, OH [1 ]
机构
[1] UNIV LIVERPOOL,PHYSIOL LAB,MRC SECRETORY CONTROL,RES GRP,LIVERPOOL L69 3BX,MERSEYSIDE,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1016/S0092-8674(00)81292-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In pancreatic acinar cells low (physiological) agonist concentrations evoke cytosolic Ca2+ spikes specifically in the apical secretory pole that contains a high density of secretory (zymogen) granules (ZGs). Inositol 1,4,5-trisphosphate (IP3) is believed to release Ca2+ from the endoplasmic reticulum, but we have now tested whether the Ca2+-releasing messengers IP3 and cyclic ADP-ribose (cADPr) can liberate Ca2+ from ZGs. In experiments on single isolated ZGs, we show using confocal microscopy that IP3 and cADPr evoke a marked decrease in the free intragranular Ca2+ concentration. Using a novel high resolution method, we have measured changes in the Ca2+ concentration in the vicinity of an isolated ZG and show that IP3 and cADPr cause rapid Ca2+ release from the granule, explaining the agonist-evoked cytosolic Ca2+ rise in the secretory pole.
引用
收藏
页码:473 / 480
页数:8
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