Derivation and Long-Term Culture of Transgene-Free Human Induced Pluripotent Stem Cells on Synthetic Substrates

被引：15
作者：
Villa-Diaz, Luis Gerardo [1 ,2 ]
Kim, Jin Koo [1 ,2 ]
Lahann, Ierg [2 ,3 ]
Krebsbach, Paul H. [1 ,2 ]
机构：
[1] Univ Michigan, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Biointerfaces Inst, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
来源：
STEM CELLS TRANSLATIONAL MEDICINE | 2014年 / 3卷 / 12期
关键词：
Induced pluripotent stem cells; Synthetic coating; Stem cells; Regenerative medicine; iPSC; Differentiation; Feeder-free culture; FEEDER-FREE CULTURE; DIRECTED DIFFERENTIATION; DEFINED CONDITIONS; POLYMER COATINGS; SENDAI-VIRUS; GROWTH; VECTOR; FIBROBLASTS; INDUCTION; SYSTEM;
D O I：
10.5966/sctm.2014-0087
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous in vitro culture on PMEDSAH-grafted plates. Together, these data demonstrate the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine.
引用
收藏
页码：1410 / 1417
页数：8
相关论文
共 27 条
[1]   Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage [J].
Amps, Katherine ;
Andrews, Peter W. ;
Anyfantis, George ;
Armstrong, Lyle ;
Avery, Stuart ;
Baharvand, Hossein ;
Baker, Julie ;
Baker, Duncan ;
Munoz, Maria B. ;
Beil, Stephen ;
Benvenisty, Nissim ;
Ben-Yosef, Dalit ;
Biancotti, Juan-Carlos ;
Bosman, Alexis ;
Brena, Romulo Martin ;
Brison, Daniel ;
Caisander, Gunilla ;
Camarasa, Maria V. ;
Chen, Jieming ;
Chiao, Eric ;
Choi, Young Min ;
Choo, Andre B. H. ;
Collins, Daniel ;
Colman, Alan ;
Crook, Jeremy M. ;
Daley, George Q. ;
Dalton, Anne ;
De Sousa, Paul A. ;
Denning, Chris ;
Downie, Janet ;
Dvorak, Petr ;
Montgomery, Karen D. ;
Feki, Anis ;
Ford, Angela ;
Fox, Victoria ;
Fraga, Ana M. ;
Frumkin, Tzvia ;
Ge, Lin ;
Gokhale, Paul J. ;
Golan-Lev, Tamar ;
Gourabi, Hamid ;
Gropp, Michal ;
Lu Guangxiu ;
Hampl, Ales ;
Harron, Katie ;
Healy, Lyn ;
Herath, Wishva ;
Holm, Frida ;
Hovatta, Outi ;
Hyllner, Johan .
NATURE BIOTECHNOLOGY, 2011, 29 (12) :1132-U113
[2]   Adaptation to culture of human embryonic stem cells and oncogenesis in vivo [J].
Baker, Duncan E. C. ;
Harrison, Neil J. ;
Maltby, Edna ;
Smith, Kath ;
Moore, Harry D. ;
Shaw, Pamela J. ;
Heath, Paul R. ;
Holden, Hazel ;
Andrews, Peter W. .
NATURE BIOTECHNOLOGY, 2007, 25 (02) :207-215
[3]  
Chen GK, 2011, NAT METHODS, V8, P424, DOI [10.1038/NMETH.1593, 10.1038/nmeth.1593]
[4]   Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation [J].
Deb-Rinker, P ;
Ly, D ;
Jezierski, A ;
Sikorska, M ;
Walker, PR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (08) :6257-6260
[5]   High Resolution Array-CGH Characterization of Human Stem Cells Using a Stem Cell Focused Microarray [J].
Elliott, Aaron M. ;
Elliott, Kristi A. Hohenstein ;
Kammesheidt, Anja .
MOLECULAR BIOTECHNOLOGY, 2010, 46 (03) :234-242
[6]   Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome [J].
Fusaki, Noemi ;
Ban, Hiroshi ;
Nishiyama, Akiyo ;
Saeki, Koichi ;
Hasegawa, Mamoru .
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 2009, 85 (08) :348-362
[7]  
Jia FJ, 2010, NAT METHODS, V7, P197, DOI [10.1038/NMETH.1426, 10.1038/nmeth.1426]
[8]   Generation of Human Induced Pluripotent Stem Cells by Direct Delivery of Reprogramming Proteins [J].
Kim, Dohoon ;
Kim, Chun-Hyung ;
Moon, Jung-Il ;
Chung, Young-Gie ;
Chang, Mi-Yoon ;
Han, Baek-Soo ;
Ko, Sanghyeok ;
Yang, Eungi ;
Cha, Kwang Yul ;
Lanza, Robert ;
Kim, Kwang-Soo .
CELL STEM CELL, 2009, 4 (06) :472-476
[9]   A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression [J].
Li, HO ;
Zhu, YF ;
Asakawa, M ;
Kuma, H ;
Hirata, T ;
Ueda, Y ;
Lee, YS ;
Fukumura, M ;
Iida, A ;
Kato, A ;
Nagai, Y ;
Hasegawa, M .
JOURNAL OF VIROLOGY, 2000, 74 (14) :6564-6569
[10]   A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells [J].
Lu, Hong Fang ;
Chai, Chou ;
Lim, Tze Chiun ;
Leong, Meng Fatt ;
Lim, Jia Kai ;
Gao, Shujun ;
Lim, Kah Leong ;
Wan, Andrew C. A. .
BIOMATERIALS, 2014, 35 (09) :2816-2826
123