Protein Crystallography and Site-Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis

被引：172

作者：

Liu, Bing ^{[1
,2
]}

He, Lihui ^{[2
,3
]}

Wang, Liping ^{[1
,2
]}

Li, Tao ^{[2
,3
]}

Li, Changcheng ^{[2
,3
]}

Liu, Huayi ^{[1
,2
]}

Luo, Yunzi ^{[1
,2
]}

Bao, Rui ^{[2
,3
]}

机构：

[1] Sichuan Univ, West China Hosp, Dept Gastroenterol, Chengdu 610041, Sichuan, Peoples R China

[2] Collaborat Innovat Ctr Biotherapy, Chengdu 610041, Sichuan, Peoples R China

[3] Sichuan Univ, West China Hosp, Ctr Infect Dis, Chengdu 610041, Sichuan, Peoples R China

来源：

CHEMBIOCHEM | 2018年 / 19卷 / 14期

基金：

中国国家自然科学基金;

关键词：

mutagenesis; polymers; protein engineering; sustainable chemistry; waste disposal; CUTINASE; CARBOXYLESTERASE; HYDROLYSIS; ESTERASES; PLASTICS; ENZYMES; LIPASE;

D O I：

10.1002/cbic.201800097

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing of poly(ethylene terephthalate) (PET) waste. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET and is, thus, a prominent candidate for PET degradation. On the basis of biochemical analysis, we propose that a wide substrate-binding pocket is critical for its excellent ability to hydrolyze crystallized PET. Structure-guided site-directed mutagenesis revealed an improvement in PETase catalytic efficiency, providing valuable insight into how the molecular engineering of PETase can optimize its application in biocatalysis.

引用

页码：1471 / 1475

页数：5

共 6 条

[1] Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
Fujiwara, Ryoga
Sanuki, Rikako
Ajiro, Hiroharu
Fukui, Toshiaki
Yoshida, Shosuke
SCIENTIFIC REPORTS, 2021, 11 (01)
[2] Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis (vol 11, 19991, 2021)
Fujiwara, Ryoga
Sanuki, Rikako
Ajiro, Hiroharu
Fukui, Toshiaki
Yoshida, Shosuke
SCIENTIFIC REPORTS, 2024, 14 (01):
[3] Development of a Targeted Gene Disruption System in the Poly(Ethylene Terephthalate)-Degrading Bacterium Ideonella sakaiensis and Its Applications to PETase and MHETase Genes
Hachisuka, Shin-ichi
Nishii, Tarou
Yoshida, Shosuke
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2021, 87 (18) : 1 - 12
[4] Ideonella sakaiensis sp nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate)
Tanasupawat, Somboon
Takehana, Toshihiko
Yoshida, Shosuke
Hiraga, Kazumi
Oda, Kohei
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2016, 66 : 2813 - 2818
[5] Characterization of the polyhydroxyalkanoate (PHA) synthase from Ideonella sakaiensis, a bacterium that is capable of degrading and assimilating poly(ethylene terephthalate)
Tan, Hua Tiang
Chek, Min Fey
Neoh, Soon Zher
Ang, Shaik Ling
Yoshida, Shosuke
Hakoshima, Toshio
Sudesh, Kumar
POLYMER DEGRADATION AND STABILITY, 2022, 206
[6] Structure-guided discovery and rational design of a new poly(ethylene terephthalate) hydrolase from AlphaFold protein structure database
Liu, Wei
Li, Chuang
Li, Bin
Zhu, Liying
Ming, Dengming
Jiang, Ling
JOURNAL OF HAZARDOUS MATERIALS, 2024, 480

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