Mastering Complexity: Towards Bottom-up Construction of Multifunctional Eukaryotic Synthetic Cells

被引：191

作者：

Goepfrich, Kerstin ^{[1
,2
]}

Platzman, Ilia ^{[1
,2
]}

Spatz, Joachim P. ^{[1
,2
]}

机构：

[1] Max Planck Inst Med Res, Dept Cellular Biophys, Jahnstr 29, D-69120 Heidelberg, Germany

[2] Heidelberg Univ, Dept Biophys Chem, Neuenheimer Feld 253, D-69120 Heidelberg, Germany

来源：

TRENDS IN BIOTECHNOLOGY | 2018年 / 36卷 / 09期

基金：

欧洲研究理事会; 欧盟地平线“2020”;

关键词：

ARTIFICIAL CELLS; MEMBRANE CHANNELS; ATP SYNTHESIS; DNA; LIPOSOMES; MICROFLUIDICS; VESICLES; MOTOR; PHOSPHORYLATION; EXPRESSION;

D O I：

10.1016/j.tibtech.2018.03.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro-disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of functional modules for synthetic cells, their classification and integration become increasingly important. We discuss strategies to reverse-engineer and recombine functional parts for synthetic eukaryotes, mimicking the characteristics of nature's own prototype. Particularly, we focus on large outer compartments, complex endomembrane systems with organelles, and versatile cytoskeletons as hallmarks of eukaryotic life. Moreover, we identify microfluidics and DNA nanotechnology as two technologies that can integrate these functional modules into sophisticated multifunctional synthetic cells.

引用

页码：938 / 951

页数：14

共 86 条

[61] ATP synthesis by the F0F1 ATP synthase from thermophilic Bacillus PS3 reconstituted into liposomes with bacteriorhodopsin .1. Factors defining the optimal reconstitution of ATP synthases with bacteriorhodopsin [J].