Selective Detection of HbA1c Using Surface Enhanced Resonance Raman Spectroscopy

被引：74

作者：

Kiran, Manikantan Syamala ^{[1
]}

Itoh, Tamitake ^{[1
]}

Yoshida, Ken-ichi ^{[1
]}

Kawashima, Nagako ^{[1
]}

Biju, Vasudevanpillai ^{[1
]}

Ishikawa, Mitsuru ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Nanobioanal Team, Hlth Technol Res Ctr, Kagawa 7610395, Japan

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 04期

关键词：

GLYCOSYLATION END-PRODUCTS; DIABETES-MELLITUS; NONENZYMATIC GLYCATION; VASCULAR-DISEASE; LIGHT-SCATTERING; HEME-PROTEINS; HEMOGLOBIN-A; GLUCOSE; NANOPARTICLES; SPECTRA;

D O I：

10.1021/ac902364h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the current work, we report on selective detection of RbA1c, a marker for glycemic control in diabetic patients, using surface enhanced resonance raman spectroscopy (SERRS). We found a characteristic band around 770-830 cm(-1) in the SERRS spectrum of HbA1c which was not present in the SERRS spectrum of HbA. To examine the contribution of glucosyl moiety to the characteristic SERRS band of RbA1c, we investigated SERRS spectra for nonenzymatically glycosylated HbA. We found that the SERRS spectral features are essentially identical for both HbA1c and nonenzymatically glycosylated HbA. Furthermore, addition of HbA into colloidal solution of silver nanoparticles (Ag NPs) resulted in the formation of large aggregates of Ag NPs and subsequent sedimentation. On the other hand, aggregation of Ag NPs was considerably low in the case of RbA1c. The differential effect of HbA and HbA1c on colloidal solution of Ag NPs, probably due to their difference in hydrophilicity, enabled us to separate them in a mixture. The separation was characterized by electrophoresis and SERRS analysis. Thus, colloidal solution of Ag NPs and SERRS would be a promising tool for the selective detection of HbA1c.

引用

页码：1342 / 1348

页数：7

共 71 条

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