Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08

被引：52

作者：

Ma, Zhao ^{[1
]}

Zhang, Chen ^{[1
]}

Gao, Xia ^{[2
]}

Cui, Fangyuan ^{[1
]}

Zhang, Jianjun ^{[1
]}

Jia, Mengshi ^{[3
]}

Jia, Shouhua ^{[4
]}

Jia, Le ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China

[2] Shandong Agr Technol Extending Stn, Jinan 250100, Shandong, Peoples R China

[3] Second High Sch Taian, Tai An 271000, Shandong, Peoples R China

[4] Shandong Agr Univ, Coll Chem & Mat Sci, Tai An 271018, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 73卷

关键词：

Flammulina velutipes SF-08; Intracellular polysaccharide; Enzymolysis and acidolysis; ANTIOXIDANT ACTIVITIES; IN-VITRO; CHEMICAL-CHARACTERIZATION; PURIFICATION; EXTRACTION; FRACTIONS; ANTITUMOR; BODIES;

D O I：

10.1016/j.ijbiomac.2014.11.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid. IPS and its derivative were purified by DEAE-52 cellulose chromatography, and five fractions were obtained. The structural features and antioxidant activities in vitro of the isolated fractions were evaluated. On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity. The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO). These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：236 / 244

页数：9

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