Interaction of phospholipid vesicles with smooth metal-oxide surfaces

被引:55
作者
Csúcs, G
Ramsden, JJ
机构
[1] Biozentrum, Dept Biophys Chem, CH-4056 Basel, Switzerland
[2] Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1998年 / 1369卷 / 01期
关键词
divalent cation; kinetics; optical waveguide lightmode spectroscopy (OWLS); phospholipid; planar bilayer; vesicle;
D O I
10.1016/S0005-2736(97)00209-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interaction of phospholipid vesicles with planar metal oxide supports has been previously reported as a means of preparing supported lipid bilayers, which are useful models of biological membranes. Nevertheless, extant evidence that bilayers are actually formed is rather circumstantial, and the necessary and sufficient conditions for their formation have never been delineated. Here, we tackle this problem by using smooth planar optical waveguides as the support. Analysis of the lightmode spectra of the waveguides, measured in situ during the deposition process, yields the mass of lipid deposited at the solid/liquid interface. By comparing the optogeometric parameters of the structures assembled from the vesicles with those of a lipid bilayer of known structure assembled using the Langmuir-Blodgett technique, we show that in many cases the vesicles remain intact and form a supported layer of vesicles rather than a bilayer, and often mixed structures (intact vesicles embedded in a bilayer partially covering the surface) occur. Careful analysis of the lipid deposition kinetics corroborates this result. We have also found that divalent cations dramatically promote attachment of mixed phosphatidylcholine/phosphatidylglycerol vesicles to form supported vesicle layers, and bilayer formation from pure phosphatidylcholine vesicles. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:61 / 70
页数:10
相关论文
共 25 条
[1]   Built-up films of barium stearate and their optical properties [J].
Blodgett, KB ;
Langmuir, I .
PHYSICAL REVIEW, 1937, 51 (11) :0964-0982
[2]   ALLOGENEIC STIMULATION OF CYTO-TOXIC T-CELLS BY SUPPORTED PLANAR MEMBRANES [J].
BRIAN, AA ;
MCCONNELL, HM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (19) :6159-6163
[3]   ELLIPSOMETRY AS A TOOL TO STUDY ADSORPTION BEHAVIOR OF SYNTHETIC AND BIOPOLYMERS AT AIR-WATER-INTERFACE [J].
DEFEIJTER, JA ;
BENJAMINS, J ;
VEER, FA .
BIOPOLYMERS, 1978, 17 (07) :1759-1772
[4]   IONIZABLE SURFACE GROUP MODELS OF AQUEOUS INTERFACES [J].
HEALY, TW ;
WHITE, LR .
ADVANCES IN COLLOID AND INTERFACE SCIENCE, 1978, 9 (04) :303-345
[5]   GEOMETRY OF RANDOM SEQUENTIAL ADSORPTION [J].
HINRICHSEN, EL ;
FEDER, J ;
JOSSANG, T .
JOURNAL OF STATISTICAL PHYSICS, 1986, 44 (5-6) :793-827
[6]  
HORN RG, 1984, BIOCHIM BIOPHYS ACTA, V778, P1224
[7]  
JACKSON SM, 1986, FARADAY DISCUSS, V81, P291
[8]   STRUCTURE OF AN ADSORBED DIMYRISTOYLPHOSPHATIDYLCHOLINE BILAYER MEASURED WITH SPECULAR REFLECTION OF NEUTRONS [J].
JOHNSON, SJ ;
BAYERL, TM ;
MCDERMOTT, DC ;
ADAM, GW ;
RENNIE, AR ;
THOMAS, RK ;
SACKMANN, E .
BIOPHYSICAL JOURNAL, 1991, 59 (02) :289-294
[9]   FORMATION OF SUPPORTED PLANAR BILAYERS BY FUSION OF VESICLES TO SUPPORTED PHOSPHOLIPID MONOLAYERS [J].
KALB, E ;
FREY, S ;
TAMM, LK .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1103 (02) :307-316
[10]   The mechanism of the surface phenomena of flotation. [J].
Langmuir, I .
TRANSACTIONS OF THE FARADAY SOCIETY, 1920, 15 (03) :0062-0074