Synthesis and DNA binding/cleavage of mononuclear copper(II) phenanthroline/bipyridine proline complexes

被引：22

作者：

Reddy, Pulimamidi R. ^{[1
]}

Raju, Nomula ^{[1
]}

Manjula, Pallerla ^{[1
]}

Reddy, Karnati V. G. ^{[1
]}

机构：

[1] Osmania Univ, Dept Chem, Hyderabad 500007, Andhra Pradesh, India

来源：

CHEMISTRY & BIODIVERSITY | 2007年 / 4卷 / 07期

关键词：

D O I：

10.1002/cbdv.200790136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complexes [Cu-II(phen)(L-Pro)(H2O)]+ClO4- (1; phen = 1,10-phenanthroline) and [Cu-II-(bipy)(L-Pro)(H2O)]+ClO4- (2; bipy = 2,2'-bipyridine) were synthesized and characterized by IR, magnetic susceptibility, UV/VIS, EPR, ESI-MS, elemental analysis, and theoretical calculations. The metal center was found in a square-pyramidal geometry. UV/VIS, thermal-denaturation, and fluorescence-spectroscopic studies were conducted to assess the interaction of the complexes with CT-DNA. An intercalative mode of binding was found, with intrinsic binding constants (K-b) of 3.86 x 10(3) and 4.6 x 10(3) M-1 and Stern-Volmer quenching constants (K) of 0.15 and 0.11 for 1 and 2, respectively. Interestingly, none of the Cu-II complexes was able to cleave pUC-19 DNA, which is attributed to the absence of a Pro amide H-atom and inhibition of the formation of an OH radical from the axially coordinated H2O molecule.

引用

页码：1565 / 1577

页数：13

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