Quantitative MR imaging of targeted SPIO particles on the cell surface and comparison to flow cytometry

被引：7

作者：

Peldschus, Kersten ^{[1
]}

Schultze, Alexander ^{[2
]}

Nollau, Peter ^{[3
]}

Kaul, Michael ^{[1
]}

Schumacher, Udo ^{[4
]}

Wagener, Christoph ^{[3
]}

Adam, Gerhard ^{[1
]}

Ittrich, Harald ^{[1
]}

机构：

[1] Univ Canc Ctr Hamburg Eppendorf UCCH, Dept Diagnost & Intervent Radiol, D-20246 Hamburg, Germany

[2] Univ Canc Ctr Hamburg Eppendorf UCCH, Dept Hematol & Oncol, D-20246 Hamburg, Germany

[3] Univ Canc Ctr Hamburg Eppendorf UCCH, Dept Clin Chem, D-20246 Hamburg, Germany

[4] Univ Canc Ctr Hamburg Eppendorf UCCH, Dept Anat Expt Morphol 2, D-20246 Hamburg, Germany

来源：

MAGNETIC RESONANCE IMAGING | 2010年 / 28卷 / 04期

关键词：

Superparamagnetic iron oxide particle; Magnetic resonance relaxometry; Flow cytometry; Carcinoembryonic antigen; IRON-OXIDE PARTICLES; IN-VIVO; CARCINOEMBRYONIC ANTIGEN; MONOCLONAL-ANTIBODIES; ADHESION MOLECULE-1; TUMOR ANGIOGENESIS; CONTRAST AGENTS; MODEL SYSTEM; NANOPARTICLES; EXPRESSION;

D O I：

10.1016/j.mri.2009.12.017

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Purpose: To detect anti-CEACAM5 targeted superparamagnetic iron oxide (SPIO) particles in vitro on the cell surface by quantitative magnetic resonance (MR) imaging and to compare with flow cytometry. Materials and Methods: The monoclonal mouse antibody T84.1 and an appropriate IgG isotype antibody were conjugated to dextran-coated SPIO particles. HT29 cells expressing carcinoembryonic antigen (CEACAM5) were treated with antibody-conjugated SPIO particles. Purified cell samples were examined on a 3.0-T MR scanner using a multi-echo spin-echo sequence for MR relaxometry. Aliquots of the cell samples were further treated with a fluorescein isothiocyanate (FITC) anti-dextran antibody and an Alexa Fluor 488 anti-mouse antibody for the corresponding flow cytometry. Results: MR relaxometry revealed a dose-dependent binding of T84.1-conjugated SPIO particles with a positive correlation between R(2) relaxation rate of cell samples and SPIO particle concentration during incubation (r=0.993, P<.01). Positive correlations were also observed between R(2) relaxation rate and flow cytometry (geometric mean) with both fluorescent antibodies (r=0.972 and r=0.953, both P<.01), respectively. Conclusion: The study revealed the feasibility of quantitative MR imaging of targeted SPIO particles on the cell surface comparable to flow cytometry. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：599 / 606

页数：8

共 29 条

[1] MR molecular imaging of the Her-2/neu receptor in breast cancer cells using targeted iron oxide nanoparticles [J].

Artemov, D ;

Mori, N ;

Okollie, B ;

Bhujwalla, ZM .

MAGNETIC RESONANCE IN MEDICINE, 2003, 49 (03) :403-408

[2]

Beauchemin N, 1999, EXP CELL RES, V252, P243

[3] CEACAM1, an adhesion molecule of human granulocytes, is fucosylated by fucosyltransferase IX and interacts with DC-SIGN of dendritic cells via Lewis x residues [J].

Bogoevska, V ;

Horst, A ;

Klampe, B ;

Lucka, L ;

Wagener, C ;

Nollau, P .

GLYCOBIOLOGY, 2006, 16 (03) :197-209

[4] Magnetic resonance visualization of tumor angiogenesis by targeting neural cell adhesion molecules with the highly sensitive gadolinium-loaded apoferritin probe [J].

Crich, Simonetta Geninatti ;

Bussolati, Benedetta ;

Tei, Lorenzo ;

Grange, Cristina ;

Esposito, Giovanna ;

Lanzardo, Stefania ;

Camussi, Giovanni ;

Aime, Silvio .

CANCER RESEARCH, 2006, 66 (18) :9196-9201

[5] Limits of detection of SPIO at 3.0 T using T2* relaxometry [J].

Dahnke, H ;

Schaeffter, T .

MAGNETIC RESONANCE IN MEDICINE, 2005, 53 (05) :1202-1206

[6] Migration of iron oxide-labeled human hematopoietic progenitor cells in a mouse model:: In vivo monitoring with 1.5-T MR imaging equipment [J].

Daldrup-Link, HE ;

Rudelius, M ;

Piontek, G ;

Metz, S ;

Bräuer, R ;

Debus, G ;

Corot, C ;

Schlegel, J ;

Link, TM ;

Peschel, C ;

Rummeny, EJ ;

Oostendorp, RAJ .

RADIOLOGY, 2005, 234 (01) :197-205

[7] CEA-related cell adhesion molecule 1:: A potent angiogenic factor and a major effector of vascular endothelial growth factor [J].

Ergün, S ;

Kilic, N ;

Ziegeler, G ;

Hansen, A ;

Nollau, P ;

Götze, J ;

Wurmbach, JH ;

Horst, A ;

Weil, J ;

Fernando, M ;

Wagener, C .

MOLECULAR CELL, 2000, 5 (02) :311-320

[8] MR imaging of the her2/neu and 9.2.27 tumor antigens using immunospecific contrast agents [J].

Funovics, MA ;

Kapeller, B ;

Hoeller, C ;

Su, HS ;

Kunstfeld, R ;

Puig, S ;

Macfelda, K .

MAGNETIC RESONANCE IMAGING, 2004, 22 (06) :843-850

[9] SPECIFIC CARCINOEMBRYONIC ANTIGENS OF HUMAN DIGESTIVE SYSTEM [J].

GOLD, P ;

FREEDMAN, SO .

JOURNAL OF EXPERIMENTAL MEDICINE, 1965, 122 (03) :467-&

[10] Antibody pretargeting advances cancer radioimmunodetection and radioimmunotherapy [J].

Goldenberg, DM ;

Sharkey, RM ;

Paganelli, G ;

Barbet, J ;

Chatal, JF .

JOURNAL OF CLINICAL ONCOLOGY, 2006, 24 (05) :823-834

← 1 2 3 →