Artemisia annua L. and photoresponse: from artemisinin accumulation, volatile profile and anatomical modifications to gene expression

被引：21

作者：

Lopes, Ellen M. ^{[1
]}

Guimaraes-Dias, Fabia ^{[2
]}

Gama, Thalia do S. S. ^{[3
]}

Macedo, Arthur L. ^{[4
,9
]}

Valverde, Alessandra L. ^{[4
]}

de Moraes, Marcela C. ^{[5
]}

de Aguiar-Dias, Ana Cristina A. ^{[6
]}

Bizzo, Humberto R. ^{[7
]}

Alves-Ferreira, Marcio ^{[2
]}

Tavares, Eliana S. ^{[8
]}

Macedo, Andrea F. ^{[1
]}

机构：

[1] Fed Univ Rio De Janeiro State UNIRIO, Integrated Lab Plant Biol LIBV, Inst Biosci, Dept Bot, Ave Pasteur 458,5th Floor,Room 512, BR-22290240 Rio De Janeiro, RJ, Brazil

[2] Fed Univ Rio De Janeiro UFRJ, Lab Plant Mol Genet LGMV, Inst Biol, Dept Genet, Rio De Janeiro, RJ, Brazil

[3] Univ Sao Paulo, Dept Bot, Inst Biosci, Lab Plant Anat LAV, Sao Paulo, SP, Brazil

[4] Fluminense Fed Univ UFF, Inst Chem, Lab Nat Prod LaProMar, Niteroi, RJ, Brazil

[5] Fluminense Fed Univ UFF, Inst Chem, Lab Chromatog & Screening Strategies, Niteroi, RJ, Brazil

[6] Fed Univ Rio De Janeiro State UNIRIO, Dept Bot, Inst Biosci, Lab Plant Anat LAV, Rio De Janeiro, RJ, Brazil

[7] Brazilian Agr Res Corp Embrapa, Food Agroind, Rio De Janeiro, RJ, Brazil

[8] Fed Univ Rio De Janeiro UFRJ, Dept Bot, Inst Biol, Lab Plant Anat, Rio De Janeiro, RJ, Brazil

[9] Fed Univ Mato Grosso Sul UFMS, Fac Pharmaceut Sci Food & Nutr, Lab Nat Prod & Mass Spectrometry LaPNEM, Postgrad Program Pharm, Campo Grande, MS, Brazil

来源：

PLANT CELL REPORTS | 2020年 / 39卷 / 01期

关键词：

Artemisia annua; Artemisinin; Glandular trichome; Light quality; Volatile; LIGHT-EMITTING-DIODES; TRICHOME-SPECIFIC EXPRESSION; ESSENTIAL OIL; LEAF ANATOMY; IN-VITRO; SECONDARY METABOLITES; LIQUID-CHROMATOGRAPHY; GLANDULAR TRICHOMES; BLUE; GROWTH;

D O I：

10.1007/s00299-019-02476-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Key message Blue and yellow light affected metabolism and the morphology. Blue and red promote the DOXP/MEP pathway. ADS gene expression was increased in plants cultivated under blue, promoting artemisinin content. Artemisinin-based combination therapies are the most effective treatment for highly lethal malaria. Artemisinin is produced in small quantities in the glandular trichomes of Artemisia annua L. Our aim was to evaluate the effect of light quality in A. annua cultivated in vitro under different light qualities, considering anatomical and morphological changes, the volatile composition, artemisinin content and the expression of two key enzymes for artemisinin biosynthesis. Yellow light is related to the increase in the number of glandular trichomes and this seemed to positively affect the molecular diversity in A. annua. Yellow light-stimulated glandular trichome frequency without triggered area enhancement, whereas blue light stimulated both parameters. Blue light enhanced the thickness of the leaf epidermis. The B-promoting effect was due to increased cell size and not to increased cell numbers. Green and yellow light positively influenced the volatile diversity in the plantlets. Nevertheless, blue and red light seemed to promote the DOXP/MEP pathway, while red light stimulates MVA pathway. Amorpha-4,11-diene synthase gene expression was significantly increased in plants cultivated under blue light, and not red light, promoting artemisinin content. Our results showed that light quality, more specifically blue and yellow light, positively affected secondary metabolism and the morphology of plantlets. It seemed that steps prior to the last one in the artemisinin biosynthesis pathway could be strongly influenced by blue light. Our work provides an alternative method to increase the amount of artemisinin production in A. annua without the use of transgenic plants, by the employment of blue light.

引用

页码：101 / 117

页数：17

共 114 条

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