The metal-free hydrogenase from methanogenic archaea: evidence for a bound cofactor

被引：68
作者：
Buurman, G
Shima, S
Thauer, RK
机构：
[1] Univ Marburg, Fachbereich Biol, Max Planck Inst Terr Mikrobiol, D-35043 Marburg, Germany
[2] Univ Marburg, Fachbereich Biol, Mikrobiol Lab, D-35043 Marburg, Germany
来源：
FEBS LETTERS | 2000年 / 485卷 / 2-3期
关键词：
hydrogenase; H-2-forming; methylenetetrahydromethanopterin dehydrogenase; methanogenic archaea; Methanobacterium thermoautotrophicum; Methanococcus jannaschii; Methanopyrus kandleri;
D O I：
10.1016/S0014-5793(00)02225-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The hmd gene, which encodes the metal-free hydrogenase in methanogenic archaea, was heterologously expressed in Escherichia coli, The overproduced enzyme was completely inactive. High activity could, however, be induced by the addition of ultrafiltrate from active enzyme denatured in 8 M urea. The active fraction in the ultrafiltrate was heat-labile and migrated on gel filtration columns with an apparent molecular mass well below 1000 Da, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：200 / 204
页数：5
相关论文
共 22 条
[11]  
Geierstanger BH, 1998, ANGEW CHEM INT EDIT, V37, P3300, DOI 10.1002/(SICI)1521-3773(19981217)37:23<3300::AID-ANIE3300>3.0.CO
[12]  
2-A
[13]   N5, N-10-METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE (H-2-FORMING) FROM THE EXTREME THERMOPHILE METHANOPYRUS-KANDLERI [J].
MA, K ;
ZIRNGIBL, C ;
LINDER, D ;
STETTER, KO ;
THAUER, RK .
ARCHIVES OF MICROBIOLOGY, 1991, 156 (01) :43-48
[14]   Remarkable cleavage of molecular hydrogen without the use of metallic catalysts: a theoretical investigation [J].
Scott, AP ;
Golding, BT ;
Radom, L .
NEW JOURNAL OF CHEMISTRY, 1998, 22 (11) :1171-1173
[15]   Hydrogenation without a metal catalyst:: An ab initio study on the mechanism of the metal-free hydrogenase from Methanobacterium thermoautotrophicum [J].
Teles, JH ;
Brode, S ;
Berkessel, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (06) :1345-1346
[16]   Reactions with molecular hydrogen in microorganisms: Evidence for a purely organic hydrogenation catalyst [J].
Thauer, RK ;
Klein, AR ;
Hartmann, GC .
CHEMICAL REVIEWS, 1996, 96 (07) :3031-3042
[17]   Biochemistry of methanogenesis: a tribute to Marjory Stephenson [J].
Thauer, RK .
MICROBIOLOGY-UK, 1998, 144 :2377-2406
[18]  
VONBUNAU R, 1991, EUR J BIOCHEM, V202, P1205
[19]  
VONBUNAU R, 1994, THESIS PHILIPPS U MA
[20]   Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp nov. [J].
Wasserfallen, A ;
Nölling, J ;
Pfister, P ;
Reeve, J ;
de Macario, EC .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :43-53
123