The metal-free hydrogenase from methanogenic archaea: evidence for a bound cofactor

被引：68

作者：

Buurman, G

Shima, S

Thauer, RK

机构：

[1] Univ Marburg, Fachbereich Biol, Max Planck Inst Terr Mikrobiol, D-35043 Marburg, Germany

[2] Univ Marburg, Fachbereich Biol, Mikrobiol Lab, D-35043 Marburg, Germany

来源：

FEBS LETTERS | 2000年 / 485卷 / 2-3期

关键词：

hydrogenase; H-2-forming; methylenetetrahydromethanopterin dehydrogenase; methanogenic archaea; Methanobacterium thermoautotrophicum; Methanococcus jannaschii; Methanopyrus kandleri;

D O I：

10.1016/S0014-5793(00)02225-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hmd gene, which encodes the metal-free hydrogenase in methanogenic archaea, was heterologously expressed in Escherichia coli, The overproduced enzyme was completely inactive. High activity could, however, be induced by the addition of ultrafiltrate from active enzyme denatured in 8 M urea. The active fraction in the ultrafiltrate was heat-labile and migrated on gel filtration columns with an apparent molecular mass well below 1000 Da, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：200 / 204

页数：5

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