Lysobacter niabensis sp nov and Lysobacter niastensis sp nov., isolated from greenhouse soils in Korea

Two bacterial strains, designated GH34-4(T) and GH41-7(T), were isolated from greenhouse soil cultivated with cucumber. The bacteria were strictly aerobic, Gram-negative, rod-shaped and oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that these strains belong to the genus Lysobacter within the Gammaproteobacteria. Strain GH34-4T showed highest sequence similarity to Lysobacteryangpyeongensis GH19-3(T) (97.5 %) and Lysobacter koreensis Elael16(T) (96.4%), and strain GH41-7T showed highest sequence similarity to Lysobacter antibioticus DSM 2044(T) (97.5 %), Lysobacter enzymogenes DSM 2043(T) (97.5 %) and Lysobacter gummosus ATCC 29489(T) (97.4%). Levels of DNA-DNA relatedness indicated that strains GH34-4(T) and GH41-7(T) represented species clearly different from L. yangpyeongensis, L. antibioticus, L. enzymogenes and L. gummosus. The major cellular fatty acids of strainsGH34-4(T) and GH41-7(T) were iso-C-16:0, iso-C-15:0 and iso-C(17:1)w9c, and the major isoprenoid quinone was Q-8. The DNA G+C contents of GH34-4(T) and GH41-7(T) were 62.5 and 66.6 mol%, respectively. On the basis of the polyphasic taxonomic data presented, it is evident that each of these strains represents a novel species of the genus Lysobacter, for which the names Lysobacter niabensis sp. nov. (type strain GH34-4(T) = KACC 11587(T) = DSM 18244(T)) and Lysobacter niastensis sp. nov. (type strain GH41-7(T) = KACC 11588(T) =DSM 18481(T)) are proposed.