Cloning and mapping of resistance gene homologues in apricot (Prunus armeniaca L.)

被引：1

作者：

Soriano, J. M. ^{[1
]}

Romero, C. ^{[1
]}

Vilanova, S. ^{[1
]}

Llacer, G. ^{[1
]}

Badenes, M. L. ^{[1
]}

机构：

[1] Inst Valenciano Invest Agr, Apartado Of, Valencia 46113, Spain

来源：

PROCEEDINGS OF THE XIIITH INTERNATIONAL SYMPOSIUM ON APRICOT BREEDING AND CULTURE | 2006年 / 717期

关键词：

NBS-LRR sequences; RGAs; apricot;

D O I：

10.17660/ActaHortic.2006.717.22

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Genomic DNA sequences, sharing homology with the NBS-LRR (Nucleotide Binding Site - Leucine Rich Repeat) resistance genes, were isolated and cloned from apricot using a PCR approach with degenerate primers designed from conserved regions of the NBS domain. RGA-specific primers were developed from the consensus sequences of each apricot RGA family and were used to develop AFLP-RGA markers by means of an AFLP-modified procedure, where one standard primer is substituted by an RGA-specific primer. Using this method, 27 polymorphic markers, six of which shared homology with the TIR class of the NBS-LRR R-genes, were obtained from 17 different primer combinations. Sixteen of the 27 mapped in an apricot genetic map previously constructed from the self-pollination of the cultivar 'Lito'.

引用

页码：115 / +

页数：3

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