Engineering a self-navigated MnARK nanovaccine for inducing potent protective immunity against novel coronavirus

被引：93

作者：

Wang, Yaling ^{[1
,2
,3
]}

Xie, Yuping ^{[3
]}

Luo, Jia ^{[3
]}

Guo, Mengyu ^{[1
,2
]}

Hu, Xuhao ^{[1
,2
,4
]}

Chen, Xi ^{[1
,2
,11
]}

Chen, Ziwei ^{[1
,2
,4
]}

Lu, Xinyi ^{[1
,2
,4
]}

Mao, Lichun ^{[1
,2
,4
]}

Zhang, Kai ^{[8
]}

Wei, Liangnian ^{[3
,5
]}

Ma, Yunfei ^{[3
]}

Wang, Ruixin ^{[3
]}

Zhou, Jia ^{[3
,10
]}

He, Chunyan ^{[3
]}

Zhang, Yufang ^{[3
]}

Zhang, Ye ^{[3
]}

Chen, Sisi ^{[3
]}

Shen, Lijuan ^{[10
]}

Chen, Yun ^{[5
]}

Qiu, Nasha ^{[1
,2
]}

Liu, Ying ^{[1
,2
]}

Cui, Yanyan ^{[9
]}

Liao, Guoyang ^{[3
]}

Liu, Ye ^{[3
]}

Chen, Chunying ^{[1
,2
,4
,6
,7
]}

机构：

[1] Chinese Acad Sci, Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Natl Ctr Nanosci & Technol China, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China

[3] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, Kunming 650000, Yunnan, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] Nanjing Med Univ, Dept Immunol, Key Lab Immune Microenvironm & Dis, Nanjing 211166, Jiangsu, Peoples R China

[6] GBA Natl Inst Nanotechnol Innovat, Guangzhou 510700, Guangdong, Peoples R China

[7] Chinese Acad Med Sci, Res Unit Nanosci & Technol, Beijing 100021, Peoples R China

[8] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China

[9] Beijing Inst Technol, Sch Optoelect, Beijing Engn Res Ctr Mixed Real & Adv Display, Beijing 100081, Peoples R China

[10] Kunming Med Univ, Sch Basic Med Sci, Dept Pathol, Kunming 650500, Yunnan, Peoples R China

[11] Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450001, Peoples R China

来源：

NANO TODAY | 2021年 / 38卷

基金：

中国国家自然科学基金;

关键词：

CELLS; DNA; VACCINATION; DEFENSE; PATHWAY;

D O I：

10.1016/j.nantod.2021.101139

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Effective vaccines are vital to fight against the COVID-19 global pandemic. As a critical component of a subunit vaccine, the adjuvant is responsible for strengthening the antigen-induced immune responses. Here, we present a new nanovaccine that comprising the Receptor-Binding Domain (RBD) of spike protein and the manganese nanoadjuvant (MnARK), which induces humoral and cellular responses. Notably, even at a 5-fold lower antigen dose and with fewer injections, the MnARK vaccine immunized mice showed stronger neutralizing abilities against the infection of the pseudovirus (similar to 270-fold) and live coronavirus (> 8-fold) in vitro than that of Alum-adsorbed RBD vaccine (Alu-RBD). Furthermore, we found that the effective co-delivery of RBD antigen and MnARK to lymph nodes (LNs) elicited an increased cellular internalization and the activation of immune cells, including DCs, CD4+ and CD8+ T lymphocytes. Our findings highlight the importance of MnARK adjuvant in the design of novel coronavirus vaccines and provide a rationale strategy to design protective vaccines through promoting cellular internalization and the activation of immune-related pathways. (C) 2021 Elsevier Ltd. All rights reserved.

引用

页数：11

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