Single radial haemolysis compared to haemagglutinin inhibition and microneutralization as a correlate of protection against influenza A H3N2 in children and adolescents

被引：17

作者：

Wang, Biao ^{[1
]}

Russell, Margaret L. ^{[2
]}

Brewer, Angela ^{[3
]}

Newton, Jennifer ^{[1
]}

Singh, Pardeep ^{[1
]}

Ward, Brian J. ^{[3
]}

Loeb, Mark ^{[1
,4
,5
,6
]}

机构：

[1] McMaster Univ, Dept Pathol & Mol Med, Hamilton, ON, Canada

[2] Univ Calgary, Cumming Sch Med, Dept Community Hlth Sci, Calgary, AB, Canada

[3] McGill Univ, Res Inst, Ctr Hlth, Montreal, PQ, Canada

[4] McMaster Univ, Dept Med, Hamilton, ON, Canada

[5] McMaster Univ, Dept Clin Epidemiol & Biostat, Hamilton, ON, Canada

[6] McMaster Univ, Michael G De Groote Inst Infect Dis Res, Hamilton, ON, Canada

来源：

INFLUENZA AND OTHER RESPIRATORY VIRUSES | 2017年 / 11卷 / 03期

关键词：

children and adolescents; correlate of protection; haemagglutinin inhibition; influenza A H3N2; microneutralization; single radial haemolysis; ANTIBODY; VACCINES; NEUTRALIZATION; DIAGNOSIS; VIRUSES; EUROPE;

D O I：

10.1111/irv.12450

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

BackgroundSerum antibodies are often used as correlates of protection for influenza. Three commonly used serological assays for detecting influenza-specific serum antibodies are single radial haemolysis (SRH), haemagglutinin inhibition (HAI) and microneutralization (MN). However, here are limited data on SRH as well as HAI and MN as correlates of protection against influenza in children and adolescents. There are also limited data that compare SRH to HAI and MN. ObjectivesWe sought primarily to understand how SRH titres correlate to protection against influenza infection in children and adolescents. We also compare SRH to HAI and MN. MethodsOf 732 healthy Hutterite children and adolescents aged between 3 and 15years were enrolled from Saskatchewan and Alberta, Canada, in the 2008-2009 flu season. Blood samples were drawn from participants at baseline and between 3 and 5weeks post-vaccination. Serum antibodies against seasonal H3N2 influenza were measured by SRH, HAI and MN assays. ResultsThe estimates of protective efficacy fluctuated when the cut-off SRH values increased. The correlation between HAI and SRH titres was 0.53 (P<.01); between MN and SRH 0.82 (P<.01); and between HAI and MN 0.50 (P<.01). Sixteen per cent of participants had SRH titres below the detection limit, compared to 7% and 34% for the MN and HAI assays. ConclusionsSRH had the worst correlation with protection against seasonal H3N2 in children and adolescents compared to MN and HAI. SRH, HAI and MN titres were significantly correlated with each other. SRH was less sensitive than MN but more sensitive than HAI.

引用

页码：283 / 288

页数：6

共 21 条

[1] Hemagglutination Inhibition Antibody Titers as a Correlate of Protection for Inactivated Influenza Vaccines in Children [J].

Black, Steven ;

Nicolay, Uwe ;

Vesikari, Timo ;

Knuf, Markus ;

Del Giudice, Giuseppe ;

Della Cioppa, Giovanni ;

Tsai, Theodore ;

Clemens, Ralf ;

Rappuoli, Rino .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2011, 30 (12) :1081-1085

[2] Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine [J].

Cheng, Li-Wei ;

Huang, Sheng-Wen ;

Huang, Li-Min ;

Chang, Luan-Yin ;

Shao, Pei-Lan ;

Kiang, David ;

Wang, Jen-Ren .

JOURNAL OF VIROLOGICAL METHODS, 2012, 182 (1-2) :43-49

[3]

Cross G., 2002, SEMINARS AVIAN EXOTI

[4] Registration of influenza vaccines for children in Europe [J].

Granstrom, Marta ;

Voordouw, Albertha C. G. .

VACCINE, 2011, 29 (43) :7572-7575

[5] Effect of Influenza Vaccination of Children on Infection Rates in Hutterite Communities A Randomized Trial [J].

Loeb, Mark ;

Russell, Margaret L. ;

Moss, Lorraine ;

Fonseca, Kevin ;

Fox, Julie ;

Earn, David J. D. ;

Aoki, Fred ;

Horsman, Gregory ;

Van Caeseele, Paul ;

Chokani, Khami ;

Vooght, Mark ;

Babiuk, Lorne ;

Webby, Richard ;

Walter, Stephen D. .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2010, 303 (10) :943-950

[6]

MANCINI G, 1983, J HYG-CAMBRIDGE, V91, P157, DOI 10.1017/S0022172400060137

[7] THE RELATIONSHIP BETWEEN SINGLE RADIAL HEMOLYSIS, HEMAGGLUTINATION INHIBITION, AND VIRUS NEUTRALIZATION ASSAYS USED TO DETECT ANTIBODIES SPECIFIC FOR EQUINE INFLUENZA-VIRUSES [J].

MORLEY, PS ;

HANSON, LK ;

BOGDAN, JR ;

TOWNSEND, HGG ;

APPLETON, JA ;

HAINES, DM .

VETERINARY MICROBIOLOGY, 1995, 45 (01) :81-92

[8]

Products CFPM, 1997, NOT GUID HARM REQ IN

[9] SINGLE RADIAL HEMOLYSIS TECHNIQUE FOR MEASUREMENT OF INFLUENZA ANTIBODY [J].

RUSSELL, SM ;

MCCAHON, D ;

BEARE, AS .

JOURNAL OF GENERAL VIROLOGY, 1975, 27 (APR) :1-10

[10]

SCHILD GC, 1975, B WORLD HEALTH ORGAN, V52, P43

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