Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients

被引：39

作者：

Garcia-Obregon, Susana ^{[1
]}

Azkargorta, Mikel ^{[2
]}

Seijas, Iratxe ^{[1
,3
]}

Pilar-Orive, Javier ^{[4
,5
]}

Borrego, Francisco ^{[6
,7
]}

Elortza, Felix ^{[2
]}

Dolores Boyano, Maria ^{[8
,9
]}

Astigarraga, Itziar ^{[1
,10
,11
]}

机构：

[1] BioCruces Hlth Res Inst, Pediat Oncol Grp, Plaza Cruces 12, Baracaldo 48903, Bizkaia, Spain

[2] CIC bioGUNE, Prote Platform, CIBERehd, ProteoRed ISCIII, Bizkaia Sci & Technol Pk, Derio 48160, Spain

[3] Hosp Univ Cruces, Intens Care Serv, Baracaldo 48903, Spain

[4] BioCruces Hlth Res Inst, Pediat Crit Care Grp, Baracaldo 48903, Spain

[5] Hosp Univ Cruces, Pediat Crit Care Serv, Baracaldo 48903, Spain

[6] BioCruces Hlth Res Inst, Immunopathol Grp, Baracaldo 48903, Spain

[7] Ikerbasque, Basque Fdn Sci, Bilbao 48013, Spain

[8] BioCruces Hlth Res Inst, Tumor Markers & Chemoresistance Grp, Baracaldo 48903, Spain

[9] Univ Basque Country, UPV EHU, Dept Cell Biol & Histol, Fac Med, Leioa 48940, Bizkaia, Spain

[10] Hosp Univ Cruces, Pediat Serv, Baracaldo 48903, Spain

[11] Univ Basque Country, Dept Pediat, Fac Med, UPV EHU, Leioa 48940, Bizkaia, Spain

来源：

JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION | 2018年 / 51卷 / 04期

关键词：

ELISA; Mass spectrometry; analysis; Proteome; Sepsis; Septic shock; Serum markers; INTERNATIONAL CONSENSUS DEFINITIONS; ANTITHROMBIN-III; SEPTIC SHOCK; CLINICAL-CRITERIA; GENE; EXPRESSION; BIOMARKERS; PROTEOMICS; OUTCOMES; PLASMA;

D O I：

10.1016/j.jmii.2016.12.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background: Sepsis is a life-threatening illness with a challenging diagnosis. Current serum biomarkers are not sensitive enough for diagnosis. With the aim of finding proteins associated with sepsis, serum protein profile was compared between patients and healthy donors and serum classical inflammatory proteins were analyzed in both groups. Methods: Serum protein profiles were characterized by two-dimensional electrophoresis (2DE). Identification of the proteins was carried out by mass spectrophotometry and their validation was performed by Enzyme-Linked-lmmunoSorbent Assay (ELISA) in a cohort of 85 patients and 67 healthy donors. Seven classical inflammatory proteins were analyzed in the same cohort by ELISA: interleukin-2 receptor alpha-chain (sCD25), scavenger receptor cysteine-

引用

页码：465 / 472

页数：8

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