Optical coherence tomography for in vivo imaging of endocardial to mesenchymal transition during avian heart development

被引：4
作者：
Courchaine, Katherine [1 ]
Rugonyi, Sandra [1 ]
机构：
[1] Oregon Hlth & Sci Univ, Dept Biomed Engn, 3303 SW Bond Ave,Mail Code CH13B, Portland, OR 97239 USA
来源：
BIOMEDICAL OPTICS EXPRESS | 2019年 / 10卷 / 11期
关键词：
OUTFLOW TRACT; EXPRESSION; FLOW; HEMODYNAMICS; MORPHOLOGY; DEFECTS;
D O I：
10.1364/BOE.10.005989
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The endocardial to mesenchymal transition (EndMT) that occurs in endocardial cushions during heart development is critical for proper heart septation and formation of the heart's valves. In EndMT, cells delaminate from the endocardium and migrate into the previously acellular endocardial cushions. Optical coherence tomography (OCT) imaging uses the optical properties of tissues for contrast, and during early development OCT can differentiate cellular versus acellular tissues. Here we show that OCT can be used to non-invasively track EndMT progression in vivo in the outflow tract cushions of chicken embryos. This enables in vivo studies to elucidate factors leading to cardiac malformations. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：5989 / 5995
页数：7
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