Validation of a Rb+ Uptake Assay for the Mouse Embryonic Stem Cell-Derived Cardiomyocytes Na+, K+ ATPase

被引：0

作者：

Gill, Sikander ^{[1
]}

Gill, Rajwant ^{[1
]}

Liang, Sophia ^{[1
]}

机构：

[1] Aurora Biomed Inc, Dept R&D, Vancouver, BC V6A 1W2, Canada

来源：

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES | 2010年 / 8卷 / 01期

关键词：

D O I：

10.1089/adt.2009.0206

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human Na+, K+ ATPase, an ATP-driven ion transporter, is an emerging drug target for heart-related conditions. Three types of assays including purified enzyme, radiotracer flux, and cold Rb+ flux have been used to determine the activity of this transporter. As an alternative to primary cardiomyocytes, mouse embryonic stem cells-derived cardiomyocytes with functional expression of essential cardiac ion channels were used in the present studies. The results on its pharmacology with digitoxin and ouabain, the 2 well-known cardioglycosides, imply that these cardiomyocytes can be used as a predictive model for the identification of modulators of Na+, K+ ATPase in HTS format.

引用

页码：114 / 117

页数：4

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